Reaction mass of 1,1'-(isopropylidene)bis[3,5-dibromo-4-(2,3-dibromo-2-methylpropoxy)benzene] and 1,3-dibromo-2-(2,3-dibromo-2-methylpropoxy)-5-{2-[3,5-dibromo-4-(2,3,3-tribromo-2-methylpropoxy)phenyl]propan-2-yl}benzene

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

16 Aug - 6 Sep 2013

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: SEPA, Guidelines for the Testing of Chemicals, 2004, 202 Daphnia sp., Acute lmmobilisation Test

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: all
- Sampling method: 2 mL of test solution was taken from each test vessel at the beginning and at the end of the test
- Sample storage conditions before analysis: none

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
101.8 mg of the test substance was dissolved in 1 L test water and stirred (via magnetic stirrer) in the dark for 72 h at 500 rpm. Later the solution was filtrated using a 0.45 μm nitrocellulose membrane to make a saturated solution of the test substance in the test water as the stock solution. The test solutions were prepared by dilution of the saturated solution with test water.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: MicroBioTests lnc (ID: DM-MBT-121126)
- Age at study initiation: less than 24 hours
- Feeding during test: none

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

none

Test temperature:

20.1 - 21.5 °C

pH:

8.02 - 8.22

Dissolved oxygen:

8.29 mg/L - 9.27 mg/L

Nominal and measured concentrations:

0, 100% (nominal, saturated solution)

Details on test conditions:

TEST SYSTEM
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
Deionised water with analytical grade salts added.
CaSO4 x 2H2O: 120 mg/L
MgSO4: 120 mg/L
NaHCO3: 192 mg/L
KCl: 8 mg/L
Na2SeO3: 2 μg/L
Vitamin B12: 1 μg/L

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: light: 16 hours; dark 8 hours
- The test water was aerated prior to the start of the study

EFFECT PARAMETERS MEASURED: mobility at 0, 24 and 48 hours

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0, 1.0, 10, 100% (nominal, saturated solution)
- Results used to determine the conditions for the definitive study: EC50 >100 mg/L (nominal, saturated solution)

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

other: test material (saturated solution)

Basis for effect:

mobility

Remarks on result:

other: Measured concentrations of the test substance were below the limit of detection of 89.6 ng/mL

Details on results:

There was no acute toxicity of the saturated solution in the limit test. The EC50 of the test substance to Daphnia magna at 24h and 48h was higher than the concentration of the saturated solution (measured concentration below the LOD2 of 89.6 ng/mL) under the tested conditions.

Results with reference substance (positive control):

EC50 = 1.1 mg/L

Table 1. Immobilisation of Daphnia magna in limit test:

Treatment group	Immobilization [%]
	24 h	48 h
Control	0	0
100 mg/L (nominal, saturated solution)	0	0

Table 2. Measured concentrations of the test substance in limit test:

Treatment group	Measured concentrations [mg/L]
	0 h	48 h
Control	not detected	not detected
100 mg/L (nominal, saturated solution)	not detected	not detected

Description of key information

EC50 (48 h) > 100 mg/L (nominal) for Daphnia magna (OECD 202)

Additional information

One study is available testing the short term toxicity of Reaction mass of 1,1'-(isopropylidene)bis[3,5-dibromo-4-(2,3-dibromo-2-methylpropoxy)benzene] and 1,3-dibromo-2-(2,3-dibromo-2-methylpropoxy)-5-{2-[3,5-dibromo-4-(2,3,3-tribromo-2-methylpropoxy)phenyl]propan-2-yl}benzene to aquatic invertebrates, including analytical results. The test was conducted in a static freshwater system according to OECD guideline 202 and GLP (Jing, 2014). The test organism Daphnia magna was exposed to a single concentration of 100 mg test item/L for 48 h. The test item was found to be poorly soluble in water and therefore the test item solution was stirred and filtrated prior to test initiation, in order to prepare a saturated solution. The concentrations of the test substance during the test were analyzed using UPLC method. The measured concentrations of the test substance were all below the limit of detection (89.6 ng/mL). No effect was observed up to the tested concentration of 100 mg/L resulting in an EC50 (48 h) >100 mg/L (nominal) based on the mobility of the organisms.