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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From July 17, 2000 to March 16, 2001
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to
Guideline:
other: Royal Decree 363/1995 (BOE no. 133 of June 1995).
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain: Strauss (Cladocera, Crustacea)
- Source: University of Stirling UK
- Age at study initiation: < 24 hours
- Feeding during test: no
- Food type: Chlorella (freshwater microalgae) and seaweed extract.

ACCLIMATION
- Temperature: 18 - 22 ºC
- Type of food: Chlorella (freshwater microalgae) and seaweed extract.
- Feeding frequency: three time per week.
- Photoperiod: 16 hours light / 8 hours of darkness.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20 ± 2 ºC
pH:
7.96 - 7.97
Dissolved oxygen:
8.08 - 8.21 mg/l O2
Nominal and measured concentrations:
NOMINAL: 0.1 mg/l, 1 mg/l, 10 mg/l, 100 mg/l and 1000 mg/l
MEASURED: 0.1 mg/l, 1 mg/l, 10 mg/l, 100.1 mg/l and 1000.8 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: 40 ml tube
- Type (delete if not applicable): closed
- Material, size: 10 ml
- Aeration: no, closed with parafilm.
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- Photoperiod: continuous darkness.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: OECD 202
— Solution 1: CaCl 2 x 2H2O 11,76 g/l H2O
— Solution 2: MgSO4 x 7H2O 4,93 g/l H2O
— Solution 3: NaHCO3 2,59 g/l H2O
— Solution 4: KCl 0,23 g/l H2O

Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
ca. 1.03 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Results with reference substance (positive control):
EC50 (24 h) = 1,1 mg/l

Inhibition of mobility

Conc. mg/l % Inhibition mobility (48h)
Control 0
0.1 15
1 55
10 85
100 90
1000 100
Conclusions:
EC50 (48 h) equal to 1.03 mg/l, nominal
Executive summary:
The substance has been tested for short-term toxicity on freshwater invertebrates according to the guideline OECD 202 (1984) and Royal Decree 363/1995 (BOE no.133 of June 1995).
The freshwater crustacean Daphnia magna has been subjected to the dye at concentrations of: 0.1mg/L, 1 mg/L, 10 mg/L, 100mg/L and 1000 mg/L of active ingredient of that product. The total test duration was 48 hours.
The result obtained in this test indicates that the dye had a nominal value of EC50 (48 h) equal to 1.03 mg/L.

Description of key information

EC50 (48 h) = 1.03 mg/l

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
1.03 mg/L

Additional information

OECD 202 (1984)