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Long-term toxicity to fish

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Description of key information

Significant differences were observed for length, wet and dry weight in the 100% saturated solution compared to the control. No significant differences were observed for hatching or post-hatch survival.

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Additional information

A fish early life stage test was conducted according to OECD 210, with fathead minnow (Pimephales promelas) (Scymaris 2021). Hydrogenated rosin alcohols is a UVCB with four main constituent groups and as the substance is difficult to test, method development work was carried out prior to the study in order to determine the most appropriate way of preparing test solutions. The use of saturator columns was found to offer the most consistent results and was therefore used in the definitive study. A semi-static test was conducted, with test solutions prepared prior to each renewal using the saturator columns and renewal of test solutions every 2 - 3 days. A limit test using a 100% saturated solution was used as the saturator columns produce a limited amount of test solution and therefore the volume of test solution would have been insufficient to include a full range of test concentrations. An initial study, terminated at 5 days post-hatch due to the control groups failing the validity criteria, showed no effects for the 100% saturated solution and therefore a limit test was considered to be appropriate for the repeat test.

Test item concentrations were monitored using GC-MS. The analytical results showed that although at the start of the study the test item concentration (based on dehydroabietyl alcohol constituent) was close to the expected solubility, the fresh test solutions from other time points, particularly at later stages of the study (when collection of test solutions occurred over a longer time period), were <LOQ, and at the end of the renewal periods concentrations were all <LOQ. 

Significant differences were observed for length, wet and dry weight in the 100% saturated solution compared to the control at the end of the test. No significant effects on hatching or post-hatch survival were observed. After test solution renewals at 12 and 14 dph sub-lethal effects were observed (loss of equilibrium, hyperventilation, corkscrew swimming and lethargy, along with haemorrhaging in some fish following renewal at 14 dph). On both occasions there was recovery, and no sub-lethal effects were observed following renewal at other time points. As significant effects were observed for the 100% saturated solution, this concentration is considered to be the LOEC and no NOEC could be determined from this study.