6,8-dianilinonaphthalene-1-sulphonic acid

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

6,8-dianilinonaphthalene-1-sulphonic acid

EC Number:

204-970-8

EC Name:

6,8-dianilinonaphthalene-1-sulphonic acid

Cas Number:

129-93-1

Molecular formula:

C22H18N2O3S

IUPAC Name:

6,8-bis(phenylamino)naphthalene-1-sulfonic acid

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

yes

Remarks:

Preliminary solution in 1% Tween 80-dimethylformamide

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow Trout
- Strain: Oncorhynchus mykiss
- Source: Parkwood Trout Farm, Kent, U.K.
- Length at study initiation: 4.7 cm +- 0.3 cm
- Weight at study initiation: 1.35 g +- 0.3 g

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

Approximately 50 mg/1 as CaC03.

Test temperature:

14 °C

Nominal and measured concentrations:

nominal 10 mg/L

Details on test conditions:

Test Water: Dechlorinated tap water (Sodium thiosulphate).

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The LC50 for fish after 96 hours is greater than 10 mg/L. At 10 mg/l no effect occured.

Executive summary:

The substance was tested for acute toxicity to fish. 10 mg/1 was the highest test concentration that could be prepared due to the limited solubility of the test material in water and auxiliary solvent and having due regard to the amount of auxiliary solvent permitted in the test under the OECD Guidelines. The use of alternative solvents and surfactants did not result in the preparation of a higher test concentration. Two replicates with 10 fish each were performed. After 96 hours no mortality was observed in both groups