4-(1-ethoxyvinyl)-3,3,5,5-tetramethylcyclohexanone

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Reason / purpose for cross-reference:

reference to same study

Qualifier:

according to guideline

Guideline:

other: Inhibition control in Ready Biodegradability test performed according to OECD 301F

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Commercial name: Kephalis
Chemical Nature: 4-(1-ethoxyvinyl)-3,3,5,5-tetramethylcyclohexanone and 1-ethoxy-4-(1-ethoxyvinyl)-3,3,5,5-tetramethylcyclohexanone
Empirical Formula: C14H24O2
Molecular weight: 224.4 g/mol
CAS No.: 36306-87-3
Appearance: pale yellow liquid
Purity: 92.5% (GC, sum of two peaks)

Note:
Please note that although the substance is stated as a mixture of 4-(1-ethoxyvinyl)-3,3,5,5-tetramethylcyclohexanone and 1-ethoxy-4-(1-ethoxyvinyl)-3,3,5,5-tetramethylcyclohex-1-ene in the report, recent analytical data has determined the substance to be a mono-constituent substance (4-(1-ethoxyvinyl)-3,3,5,5-tetramethylcyclohexanone). As the chemical manufacturing process has not changed since the report was written, the recent analytical information is relevant to the batch which was tested (1-ethoxy-4-(1-ethoxyvinyl)-3,3,5,5-tetramethylcyclohex-1-ene is not present in the substance).

Test organisms (species):

activated sludge, domestic

Details on inoculum:

Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (City of Geneva) was used.
The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day.

Reference substance (positive control):

yes

Remarks:

aniline

Key result

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Comparison of respiration of flasks containing test chemical + positive control and flasks containing positive control only

Details on results:

The curves obtained with Aniline alone and with Kephalis + aniline show no significant toxic effect of Kephalis on the microorganisms at the test concentration (see illustration section).

Conclusions:

The toxicity of Kephalis to microorganism was assessed in an inhibition control of a standardised ready test performed according to OECD 301F. Kephalis did not inhibit the degradation of the positive control, aniline, at a test concentration of 100mg/L. This concentration can be considered the NOEC for the toxicity to STP microorganisms.

Description of key information

In a standardised ready biodegradability screening study (Givaudan 1996), no toxicity was observed in an inhibition control at a test concentration of 100 mg/L. This concentration can be considered as a NOEC for the toxicity to STP microorganisms.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

100 mg/L