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Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
03.04.2017 - 06.04.2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Experimental test result performed using standard test guideline
Justification for type of information:
Data is from ABITEC lab report.
Reference:
Composition 0
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Principles of method if other than guideline:
Aim of this study was to assess the short term toxicity of Disulfo copper phthalocyanine amine salt (1328 -51 -4) to aquatic algae Desmodesmus subspicatus. Study was performed according to the OECD guideline in a static system for the total exposure period of 72 hrs.
GLP compliance:
not specified
Test material information:
Composition 1
Specific details on test material used for the study:
- Name of test material: Disulfo copper phthalocyanine amine salt
- Molecular formula: C32 H14 Cu N8 O6 S2.2Na
- Molecular weight: 780.1706 g/mol
- Smiles notation: c1ccc\2c(c1)/c/3n/c-4n/c(n\c5c6c(cccc6S(=O)(=O)[O-])c/7n5[Cu]n3/c2n\c-8n/c(n7)/c9c8c(ccc9)S(=O)(=O)[O-])/c1c4cccc1.[Na+].[Na+]
- InChl: 1S/C32H16N8O6S2.Cu.2Na/c41-47(42,43)21-13-5-11-19-23(21)31-37-27-17-9-3-1-7-15(17)25(34-27)33-26-16-8-2-4-10-18(16)28(35-26)38-32-24-20(30(40-32)36-29(19)39-31)12-6-14-22(24)48(44,45)46;;;/h1-14H,(H2-2,33,34,35,36,37,38,39,40,41,42,43,44,45,46);;;/q-2;+2;2*+1/p-2
- Substance type: Organic
- Physical state: solid
Analytical monitoring:
not specified
Vehicle:
not specified
Details on test solutions:
The stock solution 100 mg/L was prepared by dissolving dark blue powder in OECD growth medium. The solution was kept in ultrasonic bath for 20 min. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Common name:
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Institute of botany of the ASCR
- Initial biomass concentration: 5x10(3) cells /ml
- Method of cultivation: No data available

ACCLIMATION - No data available
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed:
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Remarks on exposure duration:
±1 hour
Test temperature:
23±2°C
pH:
Test at highest concentration 100 mg/l: 7.9 (Change to 7.6 during tests)
Control: 8.3 (changed to 7.4 during test)
Nominal and measured concentrations:
0, 2, 4.4, 9.7, 21, 45, 100 mg/l nominal concentrations
Details on test conditions:
TEST SYSTEM
- Test vessel: 50 ml Glass vessel
- Type (delete if not applicable): closed (with air permeable stopper)
- Sample volume: 15 ml
- Initial cells density: 5x10(3) cells/ml
- No. of vessels per concentration (replicates): 3

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Continuous
- Light intensity and quality: 6000-8000 lx

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microscope with counting chamber Cyrus I or electronic particle counter.
- Other: ErC50 was calculated using non-linear regression by the software Prism 4.0
Reference substance (positive control):
yes
Remarks:
Potassium dichromate (K2Cr2O7)
Key result
Duration:
72 h
Dose descriptor:
other: ErC50
Effect conc.:
37.1 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95% CI was 36 - 38.2 mg/l
Results with reference substance (positive control):
Results with reference substance valid
- EC50: 0.75 mg/L
Reported statistics and error estimates:
ErC50 was calculated using non-linear regression by the software Prism 4.0
Validity criteria fulfilled:
yes
Conclusions:
The median effective concentration (ErC50) for the test substance Disulfo copper phthalocyanine amine salt (1328 -51 -4), in Desmodesmus subspicatus was determined to be 37.1 mg/L on the basis of effects on growth rate in a 72 hour study.
Executive summary:

Aim of this study was to assess the short term toxicity of Disulfo copper phthalocyanine amine salt (1328 -51 -4) to aquatic algae Desmodesmus subspicatus. Study was performed according to the OECD guideline in a static system for the total exposure period of 72 hrs.

Test conducted under the static system. The stock solution 100 mg/L was prepared by dissolving dark blue powder in OECD growth medium. The solution was kept in ultrasonic bath for 20 min. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. 0, 2, 4.4, 9.7, 21, 45, 100 mg/l nominal concentrations were used.

Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration ErC50 was calculated using nonlinear regression by the software Prism 4.0.

The median effective concentration (ErC50) for the test substance Disulfo copper phthalocyanine amine salt (1328 -51 -4), in Desmodesmus subspicatus was determined to be 37.1 mg/L on the basis of effects on growth rate in a 72 hour study. Based on the this value, indicates that the substance Disulfo copper phthalocyanine amine salt (1328 -51 -4) is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 as per the CLP criteria.

Description of key information

The median effective concentration (ErC50) for the test substance Disulfo copper phthalocyanine amine salt (1328 -51 -4), in Desmodesmus subspicatus was determined to be 37.1 mg/L on the basis of effects on growth rate in a 72 hour study. Based on the this value, indicates that the substance Disulfo copper phthalocyanine amine salt (1328 -51 -4) is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 as per the CLP criteria.

Key value for chemical safety assessment

EC50/LC50 for freshwater algae:
37.1 mg/L

Additional information

For determination of effect of chemical on algae study was carried out (from ABITEC lab report, 2017). Aim of this study was to assess the short term toxicity of Disulfo copper phthalocyanine amine salt (1328 -51 -4) to aquatic algae Desmodesmus subspicatus. Study was performed according to the OECD guideline in a static system for the total exposure period of 72 hrs.

Test conducted under the static system. The stock solution 100 mg/L was prepared by dissolving dark blue powder in OECD growth medium. The solution was kept in ultrasonic bath for 20 min. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. 0, 2, 4.4, 9.7, 21, 45, 100 mg/l nominal concentrations were used.

Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration ErC50 was calculated using nonlinear regression by the software Prism 4.0.

The median effective concentration (ErC50) for the test substance Disulfo copper phthalocyanine amine salt (1328 -51 -4), in Desmodesmus subspicatus was determined to be 37.1 mg/L on the basis of effects on growth rate in a 72 hour study. Based on the this value, indicates that the substance Disulfo copper phthalocyanine amine salt (1328 -51 -4) is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 as per the CLP criteria.