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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Short term toxicity to fish:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test chemical. The nominal concentration selected for the experiment were 100mg/L and Zebra fish (Danio rerio)were exposed to these concentration for 96 hours.) and the test was carried out under static condition at hardness149.5 mg of CaCO3, test temp 23°C, pH 7.1 and dissolved oxygen 7.4 mg/L. mortalities was observed during the test. No mortalities were found in the control aquaria. The lethal concentrations LC50 was determine to be 100 mg/L.It can be concluded from the value that the test substance is toxic to the fish and can be considered as "not classified" as per the classification criteria for classification of aquatic environment.

 

Long term toxicity to fish:

Based on the prediction done using theEPI Suite ECOSAR version 1.10, the long term toxicity on fish was predicted for test chemical. On the basis of effects observed in a static freshwater system, the effect concentration NOEC value for the test chemical is estimated to be 83.368 mg/l for Species for 28 days duration. Based on this value, it can be concluded that the test substance can be considered as toxic to fish at environmentally relevant concentrations and can be consider to be classified in "not classified" as per the CLP classification criteria.

 

Short term toxicity to aquatic invertebrates:

Aim of this study was to assess the short-term toxicity of test chemical to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. The stock solution 100 g/L was prepared by dissolving yellow powder in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. 5, 10, 20, 40 and 80 mg/L concentrations were used in the study. Effects on immobilisation were observed for 48 hours. Along with the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance 32.6 mg/L, in Daphnia magna was determined to be 32.6 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be hazardous and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

 

Long term toxicity to aquatic invertebrates:

Based on the prediction done using the EPI Suite ECOSAR version 1.10, the long term toxicity on aquativ invertebrate was predicted for test chemical. On the basis of effects observed in a static freshwater system, the effect concentration NOEC value for the test chemical is estimated to be 36.775 mg/l for Species for 21 days duration. Based on this value, it can be concluded that the test substance can be considered as toxic to aquatic invertebrate at environmentally relevant concentrations and can be consider to be classified in "not classified" as per the CLP classification criteria.

 

 

Toxicity to algae and cyanobacteria:

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The stock solution 200 g/L was prepared by dissolving colorless liquid in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Various concentration were used. Along with the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs. The median effective concentration (EC50) for the test substance, in algae was determined to be 59.2 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the EC50 value, which indicates that the substance is likely to be hazardous to aquatic algae and classified as aquatic chronic 3 category as per the CLP classification criteria.

 

Toxicity to microorganisms:

1. The impairment growth concentration (IGC50) of test chemical in microorganism [Tetrahymena pyriformis] in a 48 hr study on the basis of growth inhibition effect using a short-term, static protocol was determine to be 377 mg/L.

2. The effective concentration (EC50) value of test chemical in Pseudomonas putida in a 16 hr study on the basis of growth inhibition effect was observed to be 223 mg/L.

Thus based on the above effect observation, toxicity ranges from 223 mg/l to 377 mg/l.

Additional information

Summarized result for the toxicity of test chemical on the growth and mortality of aquatic life’s including fish, invertebrates, algae and microorganism were studied and are as follows:

Short term toxicity to fish:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test chemical. The nominal concentration selected for the experiment were 100mg/L and Zebra fish (Danio rerio)were exposed to these concentration for 96 hours.) and the test was carried out under static condition at hardness149.5 mg of CaCO3, test temp 23°C, pH 7.1 and dissolved oxygen 7.4 mg/L. mortalities was observed during the test. No mortalities were found in the control aquaria. The lethal concentrations LC50 was determine to be 100 mg/L.It can be concluded from the value that the test substance is toxic to the fish and can be considered as "not classified" as per the classification criteria for classification of aquatic environment.

 

Long term toxicity to fish:

Based on the prediction done using theEPI Suite ECOSAR version 1.10, the long term toxicity on fish was predicted for test chemical. On the basis of effects observed in a static freshwater system, the effect concentration NOEC value for the test chemical is estimated to be 83.368 mg/l for Species for 28 days duration. Based on this value, it can be concluded that the test substance can be considered as toxic to fish at environmentally relevant concentrations and can be consider to be classified in "not classified" as per the CLP classification criteria.

 

Short term toxicity to aquatic invertebrates:

Aim of this study was to assess the short-term toxicity of test chemical to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. The stock solution 100 g/L was prepared by dissolving yellow powder in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. 5, 10, 20, 40 and 80 mg/L concentrations were used in the study. Effects on immobilisation were observed for 48 hours. Along with the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance 32.6 mg/L, in Daphnia magna was determined to be 32.6 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be hazardous and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

 

Long term toxicity to aquatic invertebrates:

Based on the prediction done using the EPI Suite ECOSAR version 1.10, the long term toxicity on aquativ invertebrate was predicted for test chemical. On the basis of effects observed in a static freshwater system, the effect concentration NOEC value for the test chemical is estimated to be 36.775 mg/l for Species for 21 days duration. Based on this value, it can be concluded that the test substance can be considered as toxic to aquatic invertebrate at environmentally relevant concentrations and can be consider to be classified in "not classified" as per the CLP classification criteria.

 

 

Toxicity to algae and cyanobacteria:

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The stock solution 200 g/L was prepared by dissolving colorless liquid in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Various concentration were used. Along with the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs. The median effective concentration (EC50) for the test substance, in algae was determined to be 59.2 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the EC50 value, which indicates that the substance is likely to be hazardous to aquatic algae and classified as aquatic chronic 3 category as per the CLP classification criteria.

 

Toxicity to microorganisms:

In different studies, the given test chemical and structurally and functionally similar read across chemical, has been investigated for toxicity to microorganisms to a greater or lesser extent. The studies are summarized as below –

 

In the first key study a short -term, static protocol using the Tetrahymena pyriformis was reported for the test compound. The impairment growth concentration (IGC50) of test chemical in microorganism [Tetrahymena pyriformis] in a 48 hr study on the basis of growth inhibition effect using a short-term, static protocol was determine to be 377 mg/L.

 

Similar 16 hrs hrs toxicity study to microorganism was carried out for the test compound. Test conducted for 16 hrs under the static system. The effective concentration (EC50) value of test chemical in Pseudomonas putida in a 16 hr study on the basis of growth inhibition effect was observed to be 223 mg/L.

Thus based on the above effect observation, toxicity ranges from 223 mg/l to 377 mg/l.

 

Hence based on the overall effects of test chemical on the growth of aquatic life’s, chemical consider to be toxic and classified as aquatic chronic 3 as per the CLP classification criteria.