Amides, C8-18 (even-numbered), C18unsatd, N-[3-(dimethylamino)propyl]

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

96 h

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study conducted according to guideline method with restriction. Restriction: The stability of the test concentrations was not verified with analytical monitoring.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: Annex of guideline 92/69/EWG

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Amides, C8-18 even numbered, N-[3-(dimethylamino)propyl]

EC Number:

930-947-3

IUPAC Name:

Amides, C8-18 even numbered, N-[3-(dimethylamino)propyl]

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Leuciscus idus melanotus

Details on test organisms:

TEST ORGANISM
- Common name: golden orfe
- Source: Fischzucht Eggers, Hohenwestedt
- Age at study initiation: ca. 4 weeks
- Corpulence factor: 0.8 - 1.1 g/cm³
- Feeding during test: no


ACCLIMATION
- Type and amount of food: special fish food as used from breeder
- Feeding frequency: 2x/week

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

20 ± 1 °C

pH:

From test start until termination the pH is in the range of 8.1 - 8.6 in all test vessels.

Dissolved oxygen:

From test start until termination the concentration of dissolved oxygen is in the range of 5.1 - 9.9 mg/L in all test vessels.

Nominal and measured concentrations:

nominal concentrations: control, 0.1; 0.25; 0.5; 0.75; 1.0 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: glass aquaria (L: 28.5cm; H: 24 cm; D: 21 cm); loading volume during test not reported
- Aeration: slight aeration during the test
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: cologne tap water
- Intervals of water quality measurement: every 24 h


OTHER TEST CONDITIONS
- Adjustment of pH: not reportd
- Photoperiod: 12 h dark : 12 h light

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

0.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Reported statistics and error estimates:

Statistical method: linear regression from log [mg/L] to mortality [%]

Any other information on results incl. tables

Sublethal observations / clinical signs:

Mortality:

Concentration [mg/L]	Mortality [%]
Time:	24 h	48 h	72 h	96 h
Control	0	0	0	0
0.1	0	0	0	0
0.25	0	0	0	10
0.5	0	20	40	70
0.75	10	50	60	80
1.0	40	80	100	--

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Static acute toxicity test according to OECD TG 203 conditions with Leuciscus idus melanotus: LC50 (96h) = 0.4 mg/L (nominal)

Executive summary:

Static acute toxicity test according to OECD TG 203 conditions with Leuciscus idus melanotus. The test meets the validity criteria of the guideline. No analytical monitoring was made. Therefore the test results are based on nominal concentration.

LC50 (96h) = 0.4 mg/L

Lowest concentration with 100 % mortality: 1.0 mg/L

Highest concentration with 0 % mortality: 0.1 mg/L