Pregn-5-ene-3,11,20-trione, 17-hydroxy-, cyclic 3,20-bis(1,2-ethanediyl acetal)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

other: no GLP + only for 24h exposure

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

Version / remarks:

edited in May 1996

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

(17R)-17-hydroxy-10,13-dimethyl-17-(2-methyl-1,3-dioxolan-2-yl)-1,4,7,8,9,10,12,13,14,15,16,17-dodecahydrospiro[cyclopenta[a]phenanthrene-3,2'-[1,3]dioxolan]-11(2H)-one

EC Number:

616-077-4

Cas Number:

74332-34-6

Molecular formula:

C25H36O6

IUPAC Name:

(17R)-17-hydroxy-10,13-dimethyl-17-(2-methyl-1,3-dioxolan-2-yl)-1,4,7,8,9,10,12,13,14,15,16,17-dodecahydrospiro[cyclopenta[a]phenanthrene-3,2'-[1,3]dioxolan]-11(2H)-one

Sampling and analysis

Analytical monitoring:

yes

Remarks:

concentration of cetoxenone of the aqueous solution has been determined from carbon rate measured by carbon analyzer Shimadzu TOC 5050

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The daphnies used are derived from a farm made from eggs from the toxicity screening test kit "Daphtoxkit F Magna".

Study design

Test type:

not specified

Water media type:

other: mineral water suspension

Limit test:

no

Total exposure duration:

24 h

Post exposure observation period:

Visual observation after 24h of the immobilisation of Daphnies.

Test conditions

Hardness:

Not specified

Test temperature:

Ambient temperature

pH:

Not specified

Dissolved oxygen:

Not specified

Nominal and measured concentrations:

Nominal: 6.8, 3.4, 1.7, 0.85, 0.43, 0 mg/L
Measured: -

Details on test conditions:

Ambiant temperature, protection from light
Assays are made in 4 replicates

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Key result

Duration:

24 h

Dose descriptor:

other: nominal concentration inducing 5% daphnies immobilisation

Effect conc.:

ca. 6.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The most concentrated aqueous solution of cetoxetone (6.8 mg/L) triggers an immobilisation of 5% of daphnies. Cetoxenone is thus not toxic to daphnies.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based on the experimental results, a saturated aqueous solution of cetoxenone at 6.8 mg/L is not toxic to daphnies.