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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Description of key information

EC50, 48 hours, Daphnia Magna = 0.525 mg/L

NOEC, 48 hours, Daphnia Magna = 0.413 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
0.525 mg/L

Additional information

An acute toxicity study (Huntingdon Life Sciences, 2015) was conducted in Daphnia Magna to determine the effects of the test substance MTF in aquatic invertebrates. The study was conducted according to OECD test guideline 202 and EC guideline C2, and in compliance with GLP.

Groups of twenty Daphnia, less than 24 hours old, were exposed for 48 hours to MTF, at nominal concentrations of 0.209, 0.460, 1.01, 2.23 and 4.90 mg/L; highest test level approximated the limit of aqueous solubility of the test substance. Tetrahydrofuran was used as a vehicle to aid the dissolution of the test substance. The concentrations of THF in test media were determined by Gas Chromatography-Mass Spectrometry.

Observations of the Daphnia in each control, solvent control and test vessels were made after approximately 24 and 48 hours. After 48 hours, the lowest measured concentration resulting in 100% immobility was 0.667 mg/L (mean measured) and the highest concentration at which no immobilisation occurred was 0.413 mg/L (mean measured). The 48-hour EC50 was calculated to be 0.525 mg/L (95% confidence limits 0.413 - 0.667 mg/L).