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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1999-04-28 - 1999-05-27
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report date:
1999

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Version / remarks:
July, 17, 1992
Qualifier:
according to guideline
Guideline:
EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
Version / remarks:
December 1992
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
trans-4-aminocyclohexan-1-ol
EC Number:
248-492-8
EC Name:
trans-4-aminocyclohexan-1-ol
Cas Number:
27489-62-9
Molecular formula:
C6H13NO
IUPAC Name:
4-aminocyclohexanol
Specific details on test material used for the study:
Description: white powderBatch 722Purity: not indicated by sponsor; treated as 100% puretest substance storage: at room temperature in the darkExpiry Date. 2000--04-08 (allocated by NOTOX, 1 year after receipt of the test substance)

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
freshly obtained from a municipal sewage treatment plant "Waterschap de Maaskant´, ´s-Hertogenbosch, the Netherlands
Duration of test (contact time):
ca. 28 d
Initial test substance concentration
Initial conc.:
ca. 46 other: of 1000mg Trans-4-Aminocycloexanol/l
Based on:
ThCO2
Reference substance
Reference substance:
acetic acid, sodium salt

Results and discussion

Preliminary study:
Since the purity of Trans-4-Aminocyclohexanol was unkown, calculation of the theoretical TOC value was not possible. Therefore, this value was determined by analysis. prior to the biodegradation study the TOC concentration of a Trans-4-Aminocyclohexanol solution in milli-Q-water, prepared at 1000mg/l was determined.
% Degradation
Key result
Parameter:
% degradation (CO2 evolution)
Value:
ca. 0
Sampling time:
2 d
Details on results:
The TOC concentration of the 1000mg/l trans-4-Aminocyclohexanol solution was 524.1+-12.51 ppm. based on the TOC concentration the Theoretical CO2 production (ThCO2) of the Trans-4 -Amonocyclohexanol solution was calculated to be 1.92mg CO2/ml. The concentration was 46mL of 1000mg/l trans-4-Aminocyclohexanol/l (A and B) in 2 litres test medium. Hence, the theoretical CO2 production following complete degradation was 8..3mg per 2 litres for A and B. The positive control obtained 80.2mg sodium acetate (ThCO2=1.07 mg CO2/mg) resulting in a theoretical CO2 production following complete degradation of 85.8mg per 2 litres. The toxicity control contained 80.2 mg sodium acetate and 46 mL of 1000mg Trans-4-Aminocyclohexanol mg/l in 2 litres of test medium. Hence, the theoretical CO2 production following complete degradation of Trans-4-Aminocyclohexanol plus sodium acetate was 174.1 mg per 2 litres.

BOD5 / COD results

Results with reference substance:
The positive control substance was degraded at least 60% within 7 days. The total CO2 release in the blank reached a total value of 36mg CO2 per 2 litres of medium. The difference of duplicate values for %-degradation of Trans-4-Aminocyclohexanol was always less than 20. Since all criteria for acceptability of the test were met, this study was considered to be valid.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
Trans-4-Aminocyclohexanol was not readily biodegradable under the conditions of the modified Sturm test presently performed.
Executive summary:

Since the purity of Trans-4-Aminocyclohexanol was unkown, calculation of the theoretical TOC value was not possible. Therefore, this value was determined by analysis. prior to the biodegradation study the TOC concentration of a Trans-4-Aminocyclohexanol solution in milli-Q-water, prepared at 1000mg/l was determined.

The TOC concentration of the 1000mg/l trans-4-Aminocyclohexanol solution was 524.1+-12.51 ppm. Based on the TOC concentration the Theoretical CO2 production (ThCO2) of the Trans-4 -Amonocyclohexanol solution was calculated to be 1.92mg CO2/ml.

In the modified Sturm test 46ml of the Trans-4 -Amonocyclohexanol solution was tested in 2 litres test medium, corresponding to 12mg TOC/l.

The relative degradation values calculated from the measurements performed during the test period revealed no significant degradatioon of Trans-4 -Amonocyclohexanol. In the toxicity control Trans-4 -Amonocyclohexanol was fount to be not inhibitory.

Since all acceptability criteria prescribed by the protocol were me, this study was considered to be valid.

In conclusion, Trans-4 -Aminocyclohexanol was not readily biodegrable under the conditions of the modified Sturm test presently performed.