trans-4-aminocyclohexan-1-ol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999-04-22 - 199-07-26

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

Adopted 1984-4-4

Test guideline 2

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

Test guideline 3

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

trans-4-aminocyclohexan-1-ol

EC Number:

248-492-8

EC Name:

trans-4-aminocyclohexan-1-ol

Cas Number:

27489-62-9

Molecular formula:

C6H13NO

IUPAC Name:

4-aminocyclohexanol

Specific details on test material used for the study:

Description: white powderbatch 722Purity: not indicated by sponsor; treated as 100% pureTest substance storage: at room tempearture in the darkExpiry date: 2000-04-08 (allocated by NOTOX, 1 year after receipt of the test substance)

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Crustacea, Cladocera, Straus 1820

Study design

Test type:

static

Water media type:

other: M7

Remarks:

see report

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

250mg/L expressend as CaCO3

Test temperature:

18-22°C, constant within +-1°C

pH:

9.7 (T=0h); 8.2 (T=48h)

Dissolved oxygen:

9.2mg/L (T=0h); 8.4mg/L(T=48h)

Salinity:

see report

Nominal and measured concentrations:

100mg/L

Details on test conditions:

Test vessels : 100mL, all-glassMedium: ISO, prepared in milli-RO-waterNumber of daphnia: 20 per concentrationLoading. 10 per vessel containing 80mL mediumLight. 16h photoperiod dailyFeeding: no feedingAeration: No aeration of the test solutionIntroduction of daphnids: within 20min after preparation of the test solutions.

Reference substance (positive control):

yes

Remarks:

The reference test was carried out to check the sensitivity of the test system as used by NOTOX. Daphnia were exposed for a maximum of 48h to K2Cr2O7 concentrations of 0.1, 0.18, 0.32, 0.56, 1.0 and 1.8 mg/L and to a blank control.

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Conc. based on:

test mat.

Results with reference substance (positive control):

The actual responses in this reference test with K2Cr2O7 are within the ranges of the expected responses at the different concentrations. Hence, the sensitivity of this batch of D. magna was in agreement with the historical data collected at NOTOX

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the present test, trans-4-Aminocyclohexanol induced no effects in Daphnia magna exposed to a concentration of 100mg/L. Hence, the 48h-EC50 for mobility of Daphnia magna was above 100mg/L, the maximum concentration to be tested.

Executive summary:

Trans-4 -Aminocyclohexanol was completely solible at the concentration tested. A limit test was performed exposing daphnids to a trans-4 -Aminocyclohexanol concentration of 100mg/L and a blank-control. The test considered of two vessels per group containing 10 daphnids per vessel. The study did not include sampling for determination of actual exposure concentrations.

Trans-4 -Aminocyclohexanol induced no effects in Daphnia magna exposed to a concentration of 100mg/L. Hence, 48h-EC50 for morbility of Daphnia magna was above 100mg/L, the maximum concentration to be tested