L-Ascorbic acid, 3-O-ethyl-

Water solubility

Administrative data

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 830.7840 (Water Solubility)

GLP compliance:

yes

Type of method:

flask method

Test material

Test material information

Constituent 1

Reference substance name:

(5R)-5-[(1S)-1,2-dihydroxyethyl]-4-ethoxy-3-hydroxy-2,5-dihydrofuran-2-one

EC Number:

617-849-3

Cas Number:

86404-04-8

Molecular formula:

C8H12O6

IUPAC Name:

(5R)-5-[(1S)-1,2-dihydroxyethyl]-4-ethoxy-3-hydroxy-2,5-dihydrofuran-2-one

Details on test material:

Name of test material (as cited in study report): Ethyl Ascorbic acid
Physical state: White to very pale yellow crystalline powder
Test item storage: In freezer (≤ -15°C) desiccated
Stability: Stable under storage conditions until 21 April 2018 (retest date)

Results and discussion

Water solubility

Water solubility:

>= 1 000 <= g/L

Temp.:

20 °C

pH:

>= 2.1 - <= 2.2

Any other information on results incl. tables

Results

Preliminary test

The content of test item dissolved in water was estimated to be 124 g/l during the first test. It was observed visually that test material was sticking to the stirrer bar. During the second test, the content of test item dissolved in water was 698 g/l. Based on this it was decided to perform a main study at a nominal concentration just above 1000 g/l and to shake the mixtures to prevent the test item from sticking to the stirrer bar.

Main study

The results for the samples taken at 24, 48 and 72 hours are given in the table. After stirring, no undissolved material was observed. The maximum difference (MD) of the concentrations determined in the 24, 48 and 72 hour test samples was ≤ 15%. Based on this, the water solubility of the test item is given as the mean value of the analysed concentrations. In the chromatogram of the pretreated sample from the blank water mixture, a small response was observed. Concentration calculated by extrapolation was 0.0002 g/l which was considered negligible.

Stirring time [hours]	Analysed concentration [g/l]	Mean [g/l]	MD [%]	pH
24	1031	1039	8.8	2.2
48	1088			2.1
72	996			2.1

 

The results show that concentration was comparable to the nominal concentration of 1.04 -1.07E+03 g/l. It demonstrated that all test item had dissolved and that water solubility is ≥ 1E+03 g/l.

Conclusion

The flask method was applied for the determination of the water solubility of Ethyl Ascorbic acid.

The water solubility of the test item at 20°C was ≥ 1E+03 g/l.

The pH of the aqueous samples was 2.1 – 2.2.