Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: guideline study, validity criteria fulfilled;

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report Date:
2002

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Test substance: Isobutyl-tert.Butylether
- purity: 86.4%

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
- Preparation or the inoculum : The inoculum' was washed, brought to a concentration of 5 g/I dry substance and aerated during the night . 50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/I dry substance in the test.

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
30 min

Test conditions

Details on test conditions:
- Incubation time : 30 min
- Test temperature : 20 ± 2 °C
- Test vessels : Erlenmeyer-vessel (nominal volume 250 ml)
- Test volume : 250 m l
- Synthetic medium : 8 ml/vessel 100-fold concentrated OECD medium

Results and discussion

Effect concentrationsopen allclose all
Duration:
30 min
Dose descriptor:
other: EC20
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: IUCLID4 note: "m" (measured/nominal)
Duration:
30 min
Dose descriptor:
other: EC80
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: IUCLID4 note: "m" (measured/nominal)
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: IUCLID4 note: "m" (measured/nominal)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes