Registration Dossier
Registration Dossier
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EC number: 251-347-1 | CAS number: 33021-02-2
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: guideline study, validity criteria fulfilled;
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
Test material
- Reference substance name:
- Isobutyl-tert.Butylether
- IUPAC Name:
- Isobutyl-tert.Butylether
- Details on test material:
- - Test substance: Isobutyl-tert.Butylether
- purity: 86.4%
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Preparation or the inoculum : The inoculum' was washed, brought to a concentration of 5 g/I dry substance and aerated during the night . 50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/I dry substance in the test.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 30 min
Test conditions
- Details on test conditions:
- - Incubation time : 30 min
- Test temperature : 20 ± 2 °C
- Test vessels : Erlenmeyer-vessel (nominal volume 250 ml)
- Test volume : 250 m l
- Synthetic medium : 8 ml/vessel 100-fold concentrated OECD medium
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: IUCLID4 note: "m" (measured/nominal)
- Duration:
- 30 min
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: IUCLID4 note: "m" (measured/nominal)
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: IUCLID4 note: "m" (measured/nominal)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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