1-(1,1-dimethylethoxy)-2-methylpropane

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Isobutyl-tert.Butylether (IBTBE)

IUPAC Name:

Isobutyl-tert.Butylether (IBTBE)

Details on test material:

- Test substance: Isobutyl-tert.Butylether (IBTBE)
- purity: 86.4%

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

- The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory Experimental Toxicology and Ecology of BASF AG in Ludwigshafen .

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Details on test conditions:

- Test vessel : glass tubes sealed with gas unpermeable teflone caps (nominal volume 20 ml )
- Test volume : approx. 23 ml (brimful)
- Loading (animals/ml) : approx. 0,22
- Number of animals/vessel : 5
- Total number of animals/conc. : 20
- Number of replicates/conc . : 4
- Number of replicates/control : 4
- Number of replicates for the measurement of pH and 02 : 1
- Number of parallels without animals: 1(parallel 5) per test concentration and control for measurement of the pH and oxygen content at the beginning of the exposition. This parallel was thrown away after measurement. 4 per test concentration and control for concentration control analysis .
- Age of the animals at the begin of exposition : 2 - 24 h (starting with the 3rd breed of parent animals)
- Age of stock animals : 2 - 4 weeks
- Illumination : Artificial light, type warm white (e .g. OSRAM L58 W31)
- day:night rhythm = 16 : 8 h
- Light intensity: .About 1- 8 NE/(mZ•s) at a wave length of 400 - 750 nm
- Test parameter : Swimming ability of the test animals
- Check of the study and recording (swimming ability) : Visually after 0, 24 and 48 h
- Feeding : None
- Measurement of temperature : Continuously during the whole test period in a separate vessel close the test vessels .
- Measurement of pH and oxygen : After Oh in an additional replicate without daphnids and after 48h in replicate 1 of each tested concentration .

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

4.11 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

1.75 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

7 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

EC0 (48h)   =  25   mg/l nominal (*0.07=1.75 mg/l effective)
EC50 (48h)  =  58.7 mg/l nominal (*0.07=4.11 mg/l effective)
EC100 (48h) = 100   mg/l nominal (*0.07=7.0  mg/l effective)
24 h(nominal): EC0=25mg/l, EC50=60.4mg/l, EC100>100mg/l;

Applicant's summary and conclusion

Validity criteria fulfilled:

yes