4-Nonylphenol, ethoxylated

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from J-CHECK and HSDB authoritative database

Qualifier:

according to guideline

Guideline:

other: Bioaccumulation test of a chemical substance in fish or shellfish

Principles of method if other than guideline:

Bioaccumulation test of chemical 4-Nonylphenol, ethoxylated was carried out in fish Cyprinus carpio at two different concentration for 6 weeks.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material :4-Nonylphenol, ethoxylated
- Molecular formula : C17H28O2
- Molecular weight :264.406 g/mol
- Smiles notation : c1(ccc(OCCO)cc1)CCCCCCCCC
- Substance type: Organic
- Physical state: Liquid
- Other: addition molar number of ethylene oxide ca. 10
- Water solubility: ≤ 30%

Radiolabelling:

not specified

Details on sampling:

- Sampling intervals: 2W, 3 W, 4 W and 6 W ( W= weeks)

Vehicle:

not specified

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Common carp

Route of exposure:

aqueous

Test type:

static

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

6 wk

Details on test conditions:

IDENTIFICATION AND QUANTIFICATION OF PARENT COMPOUND
- Detection limits (LOD, LOQ) (indicate method of determination/calculation): Fish : 0.15 ppm

Nominal and measured concentrations:

Nominal concentration
1st Concentration area : 1.0 mg/L
2nd Concentration area : 0.1 mg/L

Reference substance (positive control):

not specified

Details on estimation of bioconcentration:

RANGE-FINDING / PRELIMINARY STUDY
- Test concentrations: TLm(48h) 11.6 ppm, Rice fish(Oryzias latipes)

Key result

Conc. / dose:

1 mg/L

Type:

BCF

Value:

9.09 L/kg

Basis:

whole body w.w.

Time of plateau:

6 wk

Calculation basis:

other: no data

Remarks on result:

other: Other details not known

Key result

Conc. / dose:

1 mg/L

Type:

BCF

Value:

16 L/kg

Basis:

whole body w.w.

Time of plateau:

6 wk

Remarks on result:

other: Other details not known

Key result

Conc. / dose:

0.1 mg/L

Type:

BCF

Value:

7.6 L/kg

Basis:

whole body w.w.

Time of plateau:

6 wk

Calculation basis:

other: no data

Remarks on result:

other: Other details not known

Key result

Conc. / dose:

0.1 mg/L

Type:

BCF

Value:

12.4 L/kg

Basis:

whole body w.w.

Time of plateau:

6 wk

Remarks on result:

other: Other details not known

		2w	3w	4w	6w
1^stconcentration area	Test substance concentration (ppm)	0.865	0.921	0.927	0.946
	Bioconcentration factor (L/kg)	9.81	9.49	12.7	11.2
		12.5	10.1	16	9.09
2^ndconcentration area	Test substance concentration (ppm)	0.103	0.101	0.0996	0.0993
	Bioconcentration factor (L/kg)	(11.1)	(12.4)	(11.3)	(9.1)
		(12.3)	(10.0)	(7.9)	(7.6)

 

(): Reference values

Validity criteria fulfilled:

not specified

Conclusions:

The test compound 4-Nonylphenol, ethoxylated was tested for its bioaccumulation in fish Cyprinus carpio and BCF values observed are between range of 9.09 to 16 L/Kg and 7.6 to 12.4 L/Kg at concentration 1 mg/L and 0.1 mg/L respectively. These values are far less than 2000 so it is concluded that test chemical is non bioaccumulative.

Executive summary:

Bioaccumulation test was conducted for 6 weeks for determination the bioconcentration factor (BCF) of substance Nonylphenol, ethoxylated (CAS no 26027-38-3) on test organism Cyprinus carpio. Recovery ratio was : Test water : 1st concentration area : 99.7 %, 2nd concentration area : 99.2 %, Fish : 76.6 %, - Limit of detection : Fish : 0.15 mg/L and nominal concentrations used in the study as - 1st Concentration area: 01 mg/L, 2nd Concentration area : 0.1 mg/L and Range finding study was carried out at - Test concentrations: TLm (48h) 11.6 ppm, in Rice fish (Oryzias latipes). Thus according to static Fish Test the BCF value for test substance Nonylphenol, ethoxylated (CAS no.26027-38-3) was observed to be 0.09-16 L/kg at dose concentration 1 mg/L and 7.6-12.4 L/kg at dose concentration 0.1 mg/L on test organism Cyprinus carpio during 6 weeks period. These BCF values are far less than 2000 and indicate that the substance Nonylphenol, ethoxylated (CAS no.26027-38-3) is not bioaccumulative in fish and food chain.

Description of key information

Bioaccumulation test was conducted for 6 weeks for determination the bioconcentration factor (BCF) of substance Nonylphenol, ethoxylated (CAS no 26027-38-3) on test organism Cyprinus carpio (J-CHECK and HSDB, 2017). Recovery ratio was : Test water : 1st concentration area : 99.7 %, 2nd concentration area : 99.2 %, Fish : 76.6 %, - Limit of detection : Fish : 0.15 mg/L and nominal concentrations used in the study as - 1st Concentration area: 01 mg/L, 2nd Concentration area : 0.1 mg/L and Range finding study was carried out at - Test concentrations: TLm (48h) 11.6 ppm, in Rice fish (Oryzias latipes). Thus according to static Fish Test the BCF value for test substance Nonylphenol, ethoxylated (CAS no.26027-38-3) was observed to be 0.09-16 L/kg at dose concentration 1 mg/L and 7.6-12.4 L/kg at dose concentration 0.1 mg/L on test organismCyprinus carpioduring 6 weeks period. These BCF values are far less than 2000 and indicate that the substance Nonylphenol, ethoxylated (CAS no.26027-38-3) is not bioaccumulative in fish and food chain.

Key value for chemical safety assessment

BCF (aquatic species):

12.4 L/kg ww

Additional information

Various experimental and predicted data for the target compound 4-Nonylphenol, ethoxylated (CAS No. 26027-38-3) and the supporting studies for its read across substance were reviewed for the bioaccumulation end point which are summarized as below:

 

In an experimental key study fromauthoritative database (J-CHECK and HSDB, 2017), bioaccumulation test was conducted for 6 weeks for determination the bioconcentration factor (BCF) of target substance Nonylphenol, ethoxylated (CAS no 26027-38-3) on test organism Cyprinus carpio. Recovery ratio was : Test water : 1st concentration area : 99.7 %, 2nd concentration area : 99.2 %, Fish : 76.6 %, - Limit of detection : Fish : 0.15 mg/L and nominal concentrations used in the study as - 1st Concentration area: 01 mg/L, 2nd Concentration area : 0.1 mg/L and Range finding study was carried out at - Test concentrations: TLm (48h) 11.6 ppm, in Rice fish (Oryzias latipes). Thus according to static Fish Test the BCF value for test substance Nonylphenol, ethoxylated (CAS no.26027-38-3) was observed to be 0.09-16 L/kg at dose concentration 1 mg/L and 7.6-12.4 L/kg at dose concentration 0.1 mg/L on test organism Cyprinus carpio during 6 weeks period.

 

Another bioaccumulation test was conducted for 6 weeks for determination the bioconcentration factor (BCF) of target chemical Nonylphenol, ethoxylated (CAS no 26027-38-3) on test organism Cyprinus carpio (J-CHECK, 2017). Recovery ratio was : Test water : 1st concentration area : 95.4 %, 2nd concentration area : 88.2 %, Fish : 85.3 %, - Limit of detection : Test water : 1st concentration area : 79 ppb, 2nd concentration area : 8.5 ppb, Fish : 0.3 ppm and nominal concentrations used in the study as - 1st Concentration area: 02 mg/L, 2nd Concentration area : 0.2 mg/L and range finding study was carried out at - Test concentrations: TLm (48h) 11.6 ppm, in Rice fish (Oryzias latipes). Thus according to static Fish Test the BCF value for test substance Nonylphenol, ethoxylated (CAS no.26027-38-3) was observed to be less than 0.2 L/kg at dose concentration 2 mg/L and less than 1.4 L/kg at dose concentration 0.2 mg/L on test organism Cyprinus carpio during 6 weeks period.

 

Using the BCFBAF Program(v3.01) of Estimation Programs Interface (EPI Suite, 2017) the bioconcentration factor (BCF) of test chemical 4-Nonylphenol, ethoxylated (CAS No. 26027 -38 -3) was predicted. The bioconcentration factor (BCF) of 4-Nonylphenol, ethoxylated was estimated to be 9.821 L/kg whole body w.w (at 25 deg C).

 

In a supporting study from authoritative database (HSDB, 2017) for the read across chemical Dodecan-1-ol, ethoxylated (CAS no. 9002-92-0),the BCF value for chemical Dodecan-1-ol, ethoxylated was 81 and it is estimated by using log Kow of 3.40 and regression derived equation.

 

For the read across chemical 2-[2-(hexyloxy)ethoxy]ethanol (CAS no. 112-59-4), the BCF value of read across chemical 2-[2-(hexyloxy)ethoxy]ethanol was estimated(HSDB, 2017)was 4 by using log Kow of 1.70 and regression derived equation.

 

On the basis of above results for target chemical4-Nonylphenol, ethoxylated(from J-CHECK, HSDB and EPI Suite, 2017) and for its read across substance from HSDB, it can be concluded that the BCF value of test substance4-Nonylphenol, ethoxylated ranges from 0.09 –12.4which does not exceed the bioconcentration threshold of 2000, indicating that the chemical 4-Nonylphenol, ethoxylated is not expected to bioaccumulate in the food chain.