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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

bioaccumulation in aquatic species: invertebrate
Type of information:
experimental study
Adequacy of study:
key study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed journal

Data source

Reference Type:
Influence of glyphosate and its formulation (Roundup) on the toxicity and bioavailability of metals to Ceriodaphnia dubia
Martin T.K. Tsui, Wen-Xiong Wang , L.M. Chu ,
Bibliographic source:
Environmental Pollution 138 (2005) 59-68

Materials and methods

Test guideline
according to guideline
other: as mentioned below
Principles of method if other than guideline:
Data is from peer reviewed journal
GLP compliance:

Test material

Constituent 1
Chemical structure
Reference substance name:
Mercury sulphate
EC Number:
EC Name:
Mercury sulphate
Cas Number:
Molecular formula:
mercury sulfate
Constituent 2
Reference substance name:
Sulfuric acid, Mercury(2+) salt(1:1)
Sulfuric acid, Mercury(2+) salt(1:1)
Details on test material:
- Name of test material: Mercury sulphate
- Molecular formula: H2O4S.Hg
- Molecular weight : 298.67 g/mol
- Smiles notation: [O-]S(=O)(=O)[O-].[Hg+2]
- InChl : InChI=1S/Hg.H2O4S/c;1-5(2,3)4/h;(H2,1,2,3,4)/q+2;/p-2
- Substance type: Solid
- Physical state: Inorganic
- Impurities (identity and concentrations): No data
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
No data available

Sampling and analysis

Details on sampling:
No data available

Test solutions

not specified
Details on preparation of test solutions, spiked fish food or sediment:
Details on preparation of test solutions or sediment (aquatic)
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: No data available
- Controls: Yes
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): No data available
- Concentration of vehicle in test medium (stock solution and final test solution(s) at different concentrations and in control(s)): No data available
- Evidence of undissolved material (e.g. precipitate, surface film, etc): No data available

Test organisms

Test organisms (species):
Ceriodaphnia dubia
Details on test organisms:
Details on test organisms
- Common name: Water flea
- Strain: No data available
- Source: Aquatic Research Organisms (Hampton, NH, USA)
- Age at study initiation (mean and range, SD): Adults Ceriodaphnia dubia
- Weight at study initiation (mean and range, SD): No data available
- Length at study initiation (length definition, mean, range and SD): No data available
- Valve height at study initiation, for shell deposition study (mean and range, SD): No data available
- Peripheral shell growth removed prior to test initiation: No data available
- Method of breeding: No data available
- Feeding during test: No data available
- Food type: Selenastrum capricornutum and yeastecerophyle
trout chow
- Amount: No data available
- Frequency: 2

- Acclimation period: No data available
- Acclimation conditions (same as test or not): No data available
- Type and amount of food:
- Feeding frequency: No data available
- Health during acclimation (any mortality observed): No data available

QUARANTINE (wild caught)
- Duration: No data available
- Health/mortality: No data available

Study design

Route of exposure:
Test type:
not specified
Water / sediment media type:
not specified
Total exposure / uptake duration:
4 h

Test conditions

No data available
Test temperature:
No data available
No data available
Dissolved oxygen:
No data available
No data available
No data available
Details on test conditions:
Details on test conditions
- Test vessel: polypropylene beaker
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 100ml
- Aeration: No data available
- Type of flow-through (e.g. peristaltic or proportional diluter): No data available
- Renewal rate of test solution (frequency/flow rate): No data available
- No. of organisms per vessel: 10 to 20
- No. of vessels per concentration (replicates): No data available
- No. of vessels per control / vehicle control (replicates): No data available
- Biomass loading rate: No data available

- Source/preparation of dilution water: No data available
- Particulate matter: No data available
- Metals: No data available
- Pesticides: No data available
- Chlorine: No data available
- Alkalinity: No data available
- Ca/mg ratio: No data available
- Conductance: No data available
- Holding medium different from test medium: No data available
- Intervals of water quality measurement: No data available
- Intervals of test medium replacement: No data available

- Adjustment of pH: 25°C
- Photoperiod: No data available
- Light intensity: No data available

- Test concentrations: No data available
- Results used to determine the conditions for the definitive study: No data available
Nominal and measured concentrations:
Nominal: 5000, 10000, 15000, 20000 L kg-1)
Reference substance (positive control):
not specified
Details on estimation of bioconcentration:
No data available

Results and discussion

Bioaccumulation factor
other: No data available
> 0 - < 5 000
other: Bioconcentration
Remarks on result:
other: environment / dose:No data available
Details on kinetic parameters:
No data available
No data available
Results with reference substance (positive control):
No data available
Details on results:
No data available
Reported statistics:
No data available

Applicant's summary and conclusion

The bioconcentration value for the Ceriodaphnia dubia was found to be (> 0 to < 5000) value when treated with Sulfuric acid, Mercury (2+) salt (1:1) (7783-35-9).
Executive summary:

The bioconcentration test was performed on Ceriodaphnia dubia when treated with Mercury (2+) salt (1:1). Adults were used and two treatments were given as glyphosate was mixed with203Hg (0.71 nM) in the

diluent water for 30 min in a polypropylene beaker. Another only metal solution was stir for 30 minutes. After 4 hours of exposure Ceriodaphnia dubia were collected and check for radioactivity.

From the above experiment it was found that the bioconcentration value for the Ceriodaphnia dubia was found to be (> 0 to < 5000) value when treated with Sulfuric acid, Mercury (2+) salt (1:1) (7783-35-9).