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Administrative data

Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Testing was conducted between 17 December 2009 and 08 April 2010.
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results. The study report was conclusive, done to a valid guideline and the study was conducted under GLP conditions.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report date:
2010

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Type of method:
HPLC estimation method
Media:
other: Not applicable

Test material

Reference
Name:
Unnamed
Type:
Constituent
Radiolabelling:
no

Study design

Test temperature:
HPLC column maintained at 35°C by thermostatically controlled column heater. Note overall result is independent of test temperature as all values relative to literature values of calibration standards only.

HPLC method

Details on study design: HPLC method:
Please see any other information on materials and method section.
Due to the nature and tabular content of this information it would be rendered less comprehensible by splitting this information between sections.






Results and discussion

Adsorption coefficientopen allclose all
Type:
Koc
Value:
17.8 - 925
Remarks on result:
other: Lower value is a limit value, i.e. <17.8
Type:
log Koc
Value:
1.25 - 2.97
Remarks on result:
other: Lower value is a limit value, i.e. <1.25

Results: HPLC method

Details on results (HPLC method):
Please see any other information on Results and method section.
Due to the nature and tabular content of this information it would be rendered less comprehensible by splitting this information between sections.

Results: Batch equilibrium or other method

Transformation products:
not measured

Any other information on results incl. tables

Calibration

The retention times of formamide and the retention times, capacity factors (k) and log10 Kocvalues for the reference standards are shown in the two following tables:

Dead Time

Retention Time (mins)

Mean Retention Time (mins)

Injection 1

Injection 2

Formamide

2.158

2.162

2.160

Standard

Retention Time (mins)

Mean Retention Time (mins)

Capacity Factor (k)

Log10k

Log10Koc

Injection 1

Injection 2

Acetanilide

2.704

2.704

2.704

0.252

-0.599

1.25

Phenol

2.754

2.756

2.755

0.275

-0.560

1.32

Atrazine

3.084

3.090

3.087

0.429

-0.367

1.81

Isoproturon

3.321

3.327

3.324

0.539

-0.269

1.86

Triadimenol

3.925

3.932

3.929

0.819

-8.69 x 10-2

2.40

Linuron

4.549

4.555

4.552

1.107

4.43 x 10-2

2.59

Naphthalene

4.283

4.286

4.285

0.984

-7.20 x 10-3

2.75

Endosulfan-diol

5.495

5.498

5.497

1.545

0.189

3.02

Fenthion

6.669

6.665

6.667

2.087

0.319

3.31

a-Endosulfan

7.760

7.766

7.763

2.594

0.414

4.09

Phenanthrene

6.811

6.821

6.816

2.156

0.334

4.09

Diclofop-methyl

7.842

7.835

7.839

2.629

0.420

4.20

DDT

15.218

15.237

15.228

6.050

0.782

5.63

Adsorption coefficient of sample

The retention times, capacity factors and log10Koc values determined for the sample are shown in the following table:

Peak
Number

Injection

Retention Time
(mins)

Capacity
 Factor (k)

Log10k

Log10Koc

Mean
 Log10Koc

1

1

2.399

0.111

-0.956

<1.25

<1.25

2

2.394

0.108

-0.965

<1.25

2

1

2.977

0.378

-0.422

1.45

1.45

2

2.971

0.375

-0.425

1.44

3

1

3.226

0.494

-0.307

1.82

1.82

2

3.221

0.491

-0.309

1.82

4

1

4.603

1.131

5.35 x 10-2

2.97

2.97

2

4.597

1.128

5.24 x 10-2

2.96

Overall log10Kocrange: <1.25 to 2.97
Adsorption coefficient: <17.8 to 925

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The adsorption coefficient (Koc) of the test material has been determined to be in the range less than 17.8 to 925, log10 Koc <1.25 to 2.97. From the chromatographic profile, log10 Koc values of <1.25 and 1.82 were attributed to the butan-2-ol and 2,2’-oxybisbutane components of the test material respectively.
Executive summary:
The adsorption coefficient (Koc) of the test material has been determined to be in the range less than 17.8 to 925, log10Koc <1.25 to 2.97, using the HPLC screening method, Method C19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008 of 30 May 2008. From the chromatographic profile,log10Kocvalues of<1.25and 1.82 were attributed to the butan-2-ol and 2,2’-oxybisbutane components of the test material respectively.