Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study according to national standards

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1987
Report Date:
1987

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: The test follows the EU Directive 79/831/EEC, Annex V, part C, which is comparable to the OECD guideline 202.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Triphenylphosphinoxid
- Analytical purity: not specified

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: test solution was added without any pretreatment.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Strain: Sraus
- Source: Daphnia magna Straus, originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2-24 hours
- Feeding during test
- Food type: Whilst changing water Brewer's yeast was given, also 1 x daily grown Green algae culture


Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.70 +/- 0.5 mmol/l
Test temperature:
18-20 °C
pH:
about 8.0
Dissolved oxygen:
7.1 - 8.6 mg/L
Nominal and measured concentrations:
The test medium of the highest test concentration, 125 mg/l (nominal) was prepared. Following this, serial dilutions of this test medium were prepared in order to obtain the desired test concentrations. A control was tested in parallel.
Nominal concentrations: 7.81, 15.6, 31.2, 62.5, 125 ml
Details on test conditions:
TEST SYSTEM
- Test vessel: flat bottom glass tubes
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to the test guideline DIN 38412, L 11
- Culture medium different from test medium:

OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime
- Light intensity: diffuse light (5 microeinstein/m*m*s) at 400 - 750 nm wave length

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility; Visually after 0, 3, 6, 24, 48 h
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
31.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
42.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
62.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion