Sodium 4-(2-methylprop-2-en-1-yl)benzenesulphonate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Justification for type of information:

Data is from experimental study report.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Principles of method if other than guideline:

Acute oral toxicity study for test chemical in rat.

GLP compliance:

no

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Sodium 3-nitrobenzenesulphonate

EC Number:

204-857-3

EC Name:

Sodium 3-nitrobenzenesulphonate

Cas Number:

127-68-4

Molecular formula:

C6H5NO5S.Na

IUPAC Name:

sodium 3-nitrobenzenesulfonate

Test material form:

solid

Details on test material:

- Name of test material : Sodium 3-nitrobenzenesulphonate
- Molecular formula : C6H4NNaO5S
- Molecular weight : 225.1556 g/mol
- Smiles notation : c1(cc(ccc1)[N+](=O)[O-])S(=O)(=O)[O-].[Na+]
- InChl : 1S/C6H5NO5S.Na/c8-7(9)5-2-1-3-6(4-5)13(10,11)12;/h1-4H,(H,10,11,12);/q;+1/p-1
- Substance type : Organic
- Physical state : Solid

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: Mean body weight was 180 and 220 g for male dose groups 5000 and 2150, respectively, and 170 g for the two female dose groups.
- Fasting period before study: 15 - 20 h before application

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

CMC (carboxymethyl cellulose)

Details on oral exposure:

VEHICLE
MAXIMUM DOSE VOLUME APPLIED: The substance was applied as a single oral dose as a 10 mL suspension.

Doses:

2150, 5000 mg/kg b.w.

No. of animals per sex per dose:

5 (sex/dose)

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes, the post-exposure period was 14 days after which the animals were sacrificed and subjected to a gross-pathological examination.

Statistics:

not specified

Results and discussion

Preliminary study:

not specified

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Mortality:

No mortality was observed at 5000 mg/kg bw.

Clinical signs:

other: Staggering was the only clinical symptom noted at the dose level of 5000 mg/kg b.w.

Gross pathology:

Gross pathology did not reveal any abnormal findings. Both sexes showed comparable results.

Other findings:

not specified

Applicant's summary and conclusion

Interpretation of results:

other: Not classified

Conclusions:

The above experimental results prove that the LD50 value for male and female rats was determined to be >5000 mg/kg b.w. Thus, test chemical is not expected to exhibit acute oral toxicity upto the dose levels mentioned in the present study

Executive summary:

Acute oral toxicity study was conducted according to OECD Guideline 401 (Acute Oral Toxicity) by using test chemical in 20 male and female Sprague-Dawley rats at the dose concentration of 2150 and 5000 mg/kg bw.

The substance was applied as a single oral dose as a 10 mL suspension in CMC (carboxymethyl cellulose). The post-exposure period was 14 days after which the animals were sacrificed and subjected to a gross-pathological examination.

No mortality was observed at 5000 mg/kg bw. Staggering was the only clinical symptom noted at the dose level of 5000 mg/kg b.w. Body weight changes was observed. Gross pathology did not reveal any abnormal findings. Both sexes showed comparable results.

Therefore, LD50 was considered to be >5000 mg/kg bw, when 20 male and female Sprague-Dawley rats were treated with test chemical via oral gavage route.