Sodium 4-(2-methylprop-2-en-1-yl)benzenesulphonate

Endpoint summary

Description of key information

Bioaccumulation: aquatic / sediment

Bioaccumulation study was conducted on test organism Bluegill sunfish (Leponis Macrochirus)for evaluating the bioconcentration factor (BCF value) of test chemical F. R. Johannsen et. al., 1988). Test fish used for the study wasBluegill sunfish (Leponis Macrochirus) obtained from Nebraska fish farmwith a mean wet weight of 4.9±1.7 g and a mean length of 67±12 mm, respectively. All fish wereacclimatized in holding tanks for atleast 30 days prior to radiotracer exposure. Fish were fed a dry pelleted ration ad libitum each day. A modified continuous-flow proportional dilution apparatus was used to establish and maintain the desired chemical concentrations in the test chambers throughout the studies. Thirty-liter aquaria with 30 to 50 fish each were used to expose the test organisms. Aerated well water (pH 7.1, hardness 35 mg/L CaCo3, dissolved oxygen >5 mg/L, temperature 18-20°C was provided to each unit at a flow rate of 5 L/hr. Water temperatures were recorded daily while dissolved oxygen was measured at least weekly throughout the study. Radioactive test chemical stock solutions were prepared once at the beginning of the studies by the addition of a sufficientamount of radiotracer and unlabeled chemical in deionized water toachieve desired concentrations. The stock solutions were refrigerated in the dark to minimize thermal and photodegradation during storage. Test chemical conc. used for the study was 0, 6.3 ± 0.3 and 60.3 ± 2.5 mg/l (Measured test concentration) and 0.5 and 50 mg/l (Nominal test concentrations), respectively. Aquaria of 30 l volume was used as a test vessel for the study.Water and fish were sampled from each aquarium after 1, 7, 10, 21 and 28 days of exposure. Fish remaining after 28 days were transferred to clean flowing water and samples taken on the following days post exposure: 1, 3, 7. Control fish and water were sampled on days 1 and 28 of exposure. On each sample day triplicate 5-mL water samples and 5 fish were collected for analysis. The fish were eviscerated and filleted to provide samples of edible tissues (muscle) and a composite of the remaining portions of the fish. Duplicate portions of muscle tissue (fillets) and individual visceral tissue (viscera and internal organs) of fish from each test group were analyzed by standard radiometric methods.Radioactivity present in aqueous samples were determined by addition of the 5-mL samples to scintillation vials containing cocktail and counted by a liquid scintillation counter. Portions of fish carcass were either combusted (14C samples) or digested (35S samples) prior to radioanalysis of the trapped 14C02 or dissolved tissue, respectively. All measurements of radioactivity were made using a Model 2112 Packard Tri-Carb Liquid Scintillation Spectrometer respectively. All data were corrected for background, dilution, quenching and counting efficiency. Minimum detection limits were established for each sample based on the size and radiotracer counting error associated with that sample. Higher detection limits generally were applicable to the visceral tissue data, which was much smaller in sample size than the muscle tissue. The mean measured concentration of 14C-residues present in the muscle tissue remained below minimum detectable limits throughout the 7 day depuration period.The bioconcentration factor (BCF value) of test chemical on Bluegill sunfish (Leponis Macrochirus) was determined to be <6.3 and <60.3, respectively, which does not exceed the bioconcentration threshold of 2000, indicating that the chemical is not expected to bioaccumulate in the food chain.

Additional information

Bioaccumulation: aquatic / sediment

Experimental key study & predicted data of the test chemical and various supporting studies for its structurally similar read across substance were reviewed were reviewed for the bioaccumulation end point which are summarized as below:

 

In an experimental key study from peer reviewed journal (F. R. Johannsen et. al., 1988),bioaccumulation experiment was conducted on test organism Bluegill sunfish (Leponis Macrochirus)for evaluating the bioconcentration factor (BCF value) of test chemical. Test fish used for the study was Bluegill sunfish (Leponis Macrochirus) obtained from Nebraska fish farm with a mean wet weight of 4.9±1.7 g and a mean length of 67±12 mm, respectively. All fish were acclimatized in holding tanks for atleast 30 days prior to radiotracer exposure. Fish were fed a dry pelleted ration ad libitum each day. A modified continuous-flow proportional dilution apparatus was used to establish and maintain the desired chemical concentrations in the test chambers throughout the studies. Thirty-liter aquaria with 30 to 50 fish each were used to expose the test organisms. Aerated well water (pH 7.1, hardness 35 mg/L CaCo3, dissolved oxygen >5 mg/L, temperature 18-20°C was provided to each unit at a flow rate of 5 L/hr. Water temperatures were recorded daily while dissolved oxygen was measured at least weekly throughout the study. Radioactive test chemical stock solutions were prepared once at the beginning of the studies by the addition of a sufficient amount of radiotracer and unlabeled chemical in deionized water to achieve desired concentrations. The stock solutions were refrigerated in the dark to minimize thermal and photodegradation during storage. Test chemical conc. used for the study was 0, 6.3 ± 0.3 and 60.3 ± 2.5 mg/l (Measured test concentration) and 0.5 and 50 mg/l (Nominal test concentrations), respectively. Aquaria of 30 l volume was used as a test vessel for the study. Water and fish were sampled from each aquarium after 1, 7, 10, 21 and 28 days of exposure. Fish remaining after 28 days were transferred to clean flowing water and samples taken on the following days post exposure: 1, 3, 7. Control fish and water were sampled on days 1 and 28 of exposure. On each sample day triplicate 5-mL water samples and 5 fish were collected for analysis. The fish were eviscerated and filleted to provide samples of edible tissues (muscle) and a composite of the remaining portions of the fish. Duplicate portions of muscle tissue (fillets) and individual visceral tissue (viscera and internal organs) of fish from each test group were analyzed by standard radiometric methods. Radioactivity present in aqueous samples were determined by addition of the 5-mL samples to scintillation vials containing cocktail and counted by a liquid scintillation counter. Portions of fish carcass were either combusted (14C samples) or digested (35S samples) prior to radioanalysis of the trapped 14C02 or dissolved tissue, respectively. All measurements of radioactivity were made using a Model 2112 Packard Tri-Carb Liquid Scintillation Spectrometer respectively. All data were corrected for background, dilution, quenching and counting efficiency. Minimum detection limits were established for each sample based on the size and radiotracer counting error associated with that sample. Higher detection limits generally were applicable to the visceral tissue data, which was much smaller in sample size than the muscle tissue. The mean measured concentration of 14C-residues present in the muscle tissue remained below minimum detectable limits throughout the 7 day depuration period. The bioconcentration factor (BCF value) of test chemical on Bluegill sunfish (Leponis Macrochirus) was determined to be <6.3 and <60.3, respectively, which does not exceed the bioconcentration threshold of 2000, indicating that the chemical is not expected to bioaccumulate in the food chain.

 

In a prediction done using the BCFBAF Program (v3.01) of Estimation Programs Interface, the bioconcentration factor (BCF) of test chemical was estimated. The bioconcentration factor (BCF) of test chemical was estimated to be 3.162 L/kg whole body w.w (at 25 deg C) which does not exceed the bio concentration threshold of 2000, indicating that the test chemical is not expected to bioaccumulate in the food chain.

 

From CompTox Chemistry Dashboard using OPERA (OPEn (quantitative) structure-activity Relationship Application)  V1.02 model in which calculation based on PaDEL descriptors (calculate molecular descriptors and fingerprints of chemical), the bioaccumulation i.e BCF for test chemical was estimated to be 6.46 dimensionless . The predicted BCF result based on the 5 OECD principles. Thus based on the result it is concluded that the test chemical is non-bioaccumulative in nature.

 

In a supporting study from authoritative database (J-CHECK, 2018) for the test chemical,bioaccumulation experiment was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemical. The study was performed according to “OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)” and other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively. Cyprinus carpio was used as a test organism for the study. Test chemical nominal conc. used for the study was 0.5 mg/land 0.05 mg/l, respectively. Analytical method involve therecovery ratio: Fish : 84.8 %, - Limit of quantitation : Test water : 1st concentration area : 17 microg/L, 2nd concentration area :1.7 microg/L, Fish : 200 ng/g. Range finding study involves the LC50 (48 hr) ≥ 500 mg/l (w/v) on Rice fish (Oryzias latipes). Lipid content of the test organism Cyprinus carpio was determined to be 3.7% at the start of exposure. The bioconcentration factor (BCF value) of test chemical on Cyprinus carpio was determined to be<= 0.4L/Kg at a conc. of 0.5 mg/l and <= 4.1 L/Kg at a conc. of 0.05 mg/l, respectively, which does not exceed the bioconcentration threshold of 2000, indicating that the test chemical is not expected to bioaccumulate in the food chain.

 

For the test chemical, bioaccumulation experiment was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemical (authoritative database, 2018). The study was performed according to other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively. Cyprinus carpio was used as a test organism for the study. Test chemical nominal conc. used for the study was 1 mg/l and 0.1 mg/l, respectively. Analytical method involve the recovery ratio: Test water: 1st concentration area: 83.9 %, 2nd concentration area: 82.4 %, Fish : 88.1 %, - Limit of detection : Fish : 0.38 ppm. Range finding study involves the TLm (48 hr) ≥ 1000 mg/l (w/v) on Rice fish (Oryzias latipes). Lipid content of the test organism Cyprinus carpio was determined to be 2.8%. The bioconcentration factor (BCF value) of test chemical on Cyprinus carpio was determined to be<= 0.43L/Kg at a conc. of 1 mg/l and<= 3.8 L/Kg at a conc. of 0.1 mg/l, respectively, which does not exceed the bioconcentration threshold of 2000, indicating that the test chemical is not expected to bioaccumulate in the food chain.

 

On the basis of above results for test chemical, it can be concluded that the BCF value of test chemical was evaluated to be upto 60.3, respectively, which does not exceed the bioconcentration threshold of 2000, indicating that the test chemical is not expected to bioaccumulate in the food chain.