Dialuminium tris[2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)-3,4,5,6-tetrachlorobenzoate]

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

20.09.2016 - 23.09.2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Justification for type of information:

Test according to OECD 201.

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Principles of method if other than guideline:

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance Dialuminium tris(2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)-3,4,5,6-tetrachlorobenzoate) according to OECD Guideline 201.

GLP compliance:

no

Specific details on test material used for the study:

- Name of test material: Dialuminium tris(2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)-3,4,5,6-tetrachlorobenzoate)
- Molecular formula: C20H4Br4Cl4O5.2/3Al
- Physical state: Solid

Analytical monitoring:

no

Details on sampling:

No data available

Vehicle:

no

Details on test solutions:

The stock solution (100 mg/L) was prepared by dissolving dark red powder in OECD growth medium. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM- Common name: Desmodesmus subspicatus
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Institute of botany of the ASCR
- Initial biomass concentration: 5x10(3) cells /ml

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Remarks on exposure duration:

±1 hour

Post exposure observation period:

No data

Hardness:

no data

Test temperature:

23±2°C

pH:

Test: 7.7 (changed to 8.0 during test)Control: 8.2 (changed to 7.5 during test)

Dissolved oxygen:

no data

Salinity:

no data

Conductivity:

no data

Nominal and measured concentrations:

0, 5, 10, 20, 40 and 80 mg/L

Details on test conditions:

TEST SYSTEM- Test vessel: 50 ml Glass vessel
- Type (delete if not applicable): closed (with air permeable stopper)
- Sample volume: 7.5 ml- Initial cells density: 5x10(3) cells/ml
- No. of vessels per concentration (replicates): 3

GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Continuous
- Light intensity and quality: 6000-8000 lx
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microscope with counting chamber Cyrus I or electronic particle counter.
- Other: ErC50 was calculated using non-linear regression by the software Prism 4.0

Reference substance (positive control):

yes

Remarks:

Potassium dichromate (K2Cr2O7)

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

25 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 95% CL: 19,5 - 32.1

Details on results:

No data available

Results with reference substance (positive control):

Results with reference substance valid- EC50: 0.69 mg/L

Reported statistics and error estimates:

No data available

Validity criteria fulfilled:

yes

Conclusions:

The EC50 for CAS 15876-58-1 was 25.0 mg/L (95% C.I.: 19.5-32.1 mg/L) after 72 hours of exposure.

Executive summary:

A freshwater algal growth inhibition exposure assessment was used to test how a range of concentrations of CAS 15876-58-1 exerts different degrees of toxic effects on the growth of Desmodesmus subspicatusunder otherwise identical test conditions. The test was performed in close resemblance to OECD guideline 201 by ABITEC in Prague, Crez Republic. The standardized protocol used to obtain the herein described results was by the testing laboratory referred to as “CSN EN ISO 8692”. The 72 hours EC50 was by the testing laboratory defined as the concentration estimated to inhibit 50 per cent of the algae cell growth compared to unexposed cells after 72 hours of exposure. The nominal concentrations used for the exposure assessment were as follows: 5, 10, 20, 40 and 80 mg/L. The positive control/reference substance used in the tested showed an expected result and gave a EC50 that corresponded to previous exposures with this chemical in Desmodesmus subspicatus. After exposure was the EC50 calculated using nonlinear regression by the software Prism 4.0 (Graphpad Software, Inc., San Diego CA). In conclusion, the EC50 for CAS 15876-58-1 was 25.0 mg/L (95% C.I.: 19.5-32.1 mg/L) after 72 hours of exposure.

Based on the EC50, Di aluminium tris [2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate] was consider as toxic and can be consider as classified in aquatic chronic 3 as per CLP classification criteria.

Description of key information

A freshwater algal growth inhibition exposure assessment was used to test how a range of concentrations of CAS 15876-58-1 exerts different degrees of toxic effects on the growth ofDesmodesmus subspicatusunder otherwise identical test conditions. The test was performed in close resemblance to OECD guideline 201 by ABITEC in Prague, Crez Republic. The standardized protocol used to obtain the herein described results was by the testing laboratory referred to as “CSN EN ISO 8692”. The 72 hours EC50 was by the testing laboratory defined as the concentration estimated to inhibit 50 per cent of the algae cell growth compared to unexposed cells after 72 hours of exposure. The nominal concentrations used for the exposure assessment were as follows: 5, 10, 20, 40 and 80 mg/L. The positive control/reference substance used in the tested showed an expected result and gave a EC50 that corresponded to previous exposures with this chemical inDesmodesmus subspicatus. After exposure was the EC50 calculated using nonlinear regression by the software Prism 4.0 (Graphpad Software, Inc., San Diego CA). In conclusion, the EC50 for CAS 15876-58-1 was 25.0 mg/L (95% C.I.: 19.5-32.1 mg/L) after 72 hours of exposure.

Based on the EC50, Di aluminium tris [2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate] was consider as toxic and can be consider as classified in aquatic chronic 3 as per CLP classification criteria.

Key value for chemical safety assessment

EC50 for freshwater algae:

25 mg/L

Additional information

A freshwater algal growth inhibition exposure assessment was used to test how a range of concentrations of CAS 15876-58-1 exerts different degrees of toxic effects on the growth ofDesmodesmus subspicatusunder otherwise identical test conditions. The test was performed in close resemblance to OECD guideline 201 by ABITEC in Prague, Crez Republic. The standardized protocol used to obtain the herein described results was by the testing laboratory referred to as “CSN EN ISO 8692”. The 72 hours EC50 was by the testing laboratory defined as the concentration estimated to inhibit 50 per cent of the algae cell growth compared to unexposed cells after 72 hours of exposure. The nominal concentrations used for the exposure assessment were as follows: 5, 10, 20, 40 and 80 mg/L. The positive control/reference substance used in the tested showed an expected result and gave a EC50 that corresponded to previous exposures with this chemical inDesmodesmus subspicatus. After exposure was the EC50 calculated using nonlinear regression by the software Prism 4.0 (Graphpad Software, Inc., San Diego CA). In conclusion, the EC50 for CAS 15876-58-1 was 25.0 mg/L (95% C.I.: 19.5-32.1 mg/L) after 72 hours of exposure.

Based on the EC50, Di aluminium tris [2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate] was consider as toxic and can be consider as classified in aquatic chronic 3 as per CLP classification criteria.