Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1986
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Raw data available, not GLP compliant
Principles of method if other than guideline:
Sludge was exposed to the test substance at nominal concentrations of 0, 10, 32 and 100 mg/L for 3 h. Bacterial toxicity was measured as inhibition of O2 consumption.
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
not specified
Details on test solutions:
- Nominal: 0, 10, 32 and 100 mg/L test substance
Test organisms (species):
activated sludge of a predominantly domestic sewage
Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol
Key result
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Key result
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
> 37.3 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
inhibition of total respiration
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- Relevant effect levels: 3 h EC50: 20 mg/L
 Test substance (mg/L)  O2 consumption (mg/L/min)  Inhibition (%)
 10 0.81 0
 32  0.92  0
 100  1.01  0
 0  0.73-0.83  -
Validity criteria fulfilled:
not specified
Conclusions:
Under the study conditions, the nominal EC10 of the test substance was >100 mg/L, i.e. >37.3 mg a.i./L.
Executive summary:

A study was conducted to determine the toxicity of the test substance (37.3% purity) to wastewater sludge bacteria according to internal method B-10. Non-adapted principally communal wastewater sludge was exposed to nominal concentrations of 10, 32 and 100 mg/L test substance, equivalent to 6.3, 20.0 and 62.5 mg a.i./L, for 3 h. The positive reference 3,5-dichlorophenol was run in parallel at 10, 32 and 100 mg/L. Under the study conditions, the nominal EC10 was >100 mg/L, i.e. >37.3 mg a.i./L (Reust, 1986).

Description of key information

Key value for chemical safety assessment

Additional information

A study was conducted to determine the toxicity of the test substance (37.3% purity) to wastewater sludge bacteria according to internal method B-10. Non-adapted principally communal wastewater sludge was exposed to nominal concentrations of 10, 32 and 100 mg/L test substance, equivalent to 6.3, 20.0 and 62.5 mg a.i./L, for 3 h. The positive reference 3,5-dichlorophenol was run in parallel at 10, 32 and 100 mg/L. Under the study conditions, the nominal EC10 was >100 mg/L, i.e. >37.3 mg a.i./L (Reust, 1986).