3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-N,N,N-trimethylanilinium chloride

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29.11.2016 – 01.12.2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

study is from experimental report

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

Short term toxicity of test chemical to aquatic invertebrates was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system.

GLP compliance:

no

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No. of test material: No data
- Expiration date of the lot/batch: No data
- Purity test date: No data

RADIOLABELLING INFORMATION (if applicable)
- Radiochemical purity: No data
- Specific activity: No data
- Locations of the label: No data
- Expiration date of radiochemical substance: No data

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: No data
- Stability under test conditions: No data
- Solubility and stability of the test substance in the solvent/vehicle: No data
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: No data

Analytical monitoring:

no

Details on sampling:

No data

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
The stock solution 100.0 mg/L was prepared by dissolving orange powder in reconstituted water. The test solutions of required concentrations were prepared by mixing the stock solution of the test sample in reconstituted water.

- Controls: Reconstituted water as specified in OECD 202, used as a control and for sample dilution.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain/clone: Straus
- Source: Own breeding at University of Chemistry and Technology, Prague
- Age: Young daphnids, aged less than 24 hours used at the start of the test and they should not be first brood progeny.
- Feeding during test : Without feeding

ACCLIMATION
- Acclimation period: No data
- Acclimation conditions (same as test or not): No data
- Type and amount of food: No data
- Feeding frequency: No data
- Health during acclimation (any mortality observed): No data

QUARANTINE (wild caught)
- Duration: No data
- Health/mortality: No data

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

± 1 h

Post exposure observation period:

No data

Hardness:

No data

Test temperature:

20 °C ± 1 °C

pH:

Sample at concentration 85.0 mg/L : pH = 7.5 changed to pH = 7.8 during the test,
Control: pH = 7.8 changed to pH = 7.7 during the test.

Dissolved oxygen:

Higher than 8.3 mg/L both in the control and the sample.

Salinity:

No data

Conductivity:

No data

Nominal and measured concentrations:

0, 10, 17, 29, 50 and 85 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 50 ml glass vessel
- Material, size, headspace, fill volume: 25 ml
- Aeration: no data
- No. of organisms per vessel: 5 pieces
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): No data
- No. of vessels per vehicle control (replicates): No data
- Biomass loading rate: No data

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural water (surface or ground water), reconstituted water or dechlorinated tap water are acceptable as culturing and dilution water if D. magna survives in it for the duration of the culturing, acclimation and testing without showing signs of stress. Waters in the range pH 6 to pH 9, with hardness between 140 mg/l and 275 mg/l (as CaCO3) are recommended.As an example, the preparation of dilution water meeting the requirements is described below.Dissolve known quantities of reagents in water. The dilution water prepared shall have a pH of 7.8 ± 0.5, a hardness of (225 ± 50) mg/l (expressed as CaCO3), a molar Ca + Mg ratio close to 4 + 1 and a dissolved oxygen concentration above 7 mg/l.

OTHER TEST CONDITIONS
- Adjustment of pH: Without adjustment
- Photoperiod: No - Darkness

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : EC50 was calculated using nonlinear regression by the software Prism 4.0

Reference substance (positive control):

yes

Remarks:

[Potassium dichromate (K2Cr2O7)]

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

35.6 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL : 23.8 – 53.3 mg/L

Details on results:

No data

Results with reference substance (positive control):

- Results with reference substance valid
- 24h EC50 = 0.79 mg/L K2Cr2O7

Reported statistics and error estimates:

No data

Control solution: Reconstituted water without the sample , 0 % of the daphnids were immobilized at the end of the test.

Validity criteria fulfilled:

yes

Conclusions:

The median effective concentration (EC50) for the test substance, 3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-N,N,N-trimethylanilinium chloride, in Daphnia magna was determined to be 35.6 mg/L on the basis of mobility inhibition effects in a 48 hour study.

Executive summary:

Determination of the inhibition of the mobility of daphnids was carried out with the substance 3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-N,N,N-trimethylanilinium chloride according to OECD Guideline 202.

The test substance was tested at the concentrations 0, 10, 17, 29, 50 and 85 mg/L. Effects on immobilisation were observed for 48 hours.

The median effective concentration (EC50) for the test substance, 3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-N,N,N-trimethyl anilinium chloride, in Daphnia magna was determined to be 35.6 mg/L and also the substance was found to be not rapidly degradable. Thus considering theCriteria for aquatic classification of the substance, it is concluded that the substance ‘3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-N,N,N-trimethyl anilinium chloride’ exhibits toxicity to aquatic invertebrate (Daphnia magna) in the chronic 3 classification category.

Description of key information

The median effective concentration (EC50) for the test substance, 3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-N,N,N-trimethyl anilinium chloride, in Daphnia magna was determined to be 35.6 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

35.6 mg/L

Additional information

Determination of the inhibition of the mobility of daphnids was carried out with the substance 3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-N,N,N-trimethylanilinium chloride according to OECD Guideline 202. The test substance was tested at the concentrations 0, 10, 17, 29, 50 and 85 mg/L. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, 3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-N,N,N-trimethyl anilinium chloride, in Daphnia magna was determined to be 35.6 mg/L and also the substance was observed to be not rapidly degradable. Thus considering the Criteria for aquatic classification of the substance, it is concluded that the substance ‘3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-N,N,N-trimethyl anilinium chloride’ exhibits toxicity to aquatic invertebrate (Daphnia magna) in the chronic 3 classification category.