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EC number: 943-097-3 | CAS number: -
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1987
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- The main structure of the read-across substance is the same of the CJ313, only the salt form is different. However, this difference does not influence the toxicity of the CJ313. CJ313 contains Li/Na salt form and the read-across substances contains only Na salt. This will not lead to difference on the toxicity results.
- Qualifier:
- according to guideline
- Guideline:
- other: CIBA Method A-11
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Principles of method if other than guideline:
- In principle the methods described in OECD Guideline 203 were used.
- GLP compliance:
- no
- Specific details on test material used for the study:
- None
- Analytical monitoring:
- yes
- Details on sampling:
- None
- Vehicle:
- yes
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Rainbow trout: (Salmo gairdneri)
Replicates: 8 per test concentration
Age: about 4-6 months
Size: 4-8 cm
Weight: 3-6 g
Supplier: P. Hohler, 4314 Zeiningen
Golden orfe: (Leuciscus idus)
Replicates: 8 per test concentration
Age: about 12 months
Size: 3-4 cm
Weight: 1.5-2.5 g
Zebrafish: (Danio rerio)
Replicates: 10 per test concentration
Age: about 4-6 months
Size: about 3 cm
Weight: 0.25-0.35 g
Supplier: West Aquarium GmbH, D-3422 Bad Lauterberg - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- None
- Post exposure observation period:
- None
- Hardness:
- None
- Test temperature:
- Zebra fish: 23 °C
- pH:
- 7.8
- Dissolved oxygen:
- None
- Salinity:
- None
- Conductivity:
- None
- Nominal and measured concentrations:
- 300 and 1000 mg/L
- Details on test conditions:
- Water:
15 liters for trout and orfe or 3 liters for zebrafish (active coal filtered). Water used according to ISO guidelines.
Basins:
Full-glass basins, 17 liters basis (36x23x26 cm) tempered (cold-room); 5 liter basins (15x20x20 cm) tempered - Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- No mortality occurred at 300 mg/L, while 2 out of 10 fish died at 1000 mg/L concentration.
- Results with reference substance (positive control):
- None
- Reported statistics and error estimates:
- None
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The LC50 of FAT 40032/D in this fish acute toxicity test was determined to be >1000 mg/L.
- Executive summary:
The acute fish toxicity test was performed according to a methodology similar to OECD Guideline 203. Zebrafish: (Danio rerio) were used in the experiment setup. Fish were tested at these different following concentrations: 320 and 1000 mg/L. No mortality occurred at 300 mg/L, while 2 out of 10 fish died at 1000 mg/L concentration.
In conclusion the results obtained were.
LC0 = 300 mg/L
LC50 >1000 mg/L
LC100 >1000 mg/L.
Reference
Description of key information
The LC50 of FAT 40032/D in the fish acute toxicity test was determined to be greater than 1000 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 1 000 mg/L
Additional information
Acute toxicity of Reactive Brown 011 to fish was determined in four available studies. The outcome of all these studies, indicated the LC50 to be >1000 mg/L.
In the key study, Zebrafish: (Danio rerio) were exposed to FAT 40032/D at 300 and 1000 mg/L for 96 hours. No mortality occurred at 300 mg/L while 2 out of 10 fish were found dead at 1000 mg/L concentration.
In conclusion the results obtained were:
LC0 = 300 mg/L
LC50 >1000 mg/L
LC100 >1000 mg/L.
Several other studies are available, results of which are presented below:
FAT 40032/A:
TL0 = 1000 mg/L
TL50 = >1000 mg/L
TL100 = >1000 mg/L
FAT 40032/B: (96 h)
TL0 = 1000 mg/L
TL50 >1000 mg/L
TL100 >1000 mg/L
FAT 40032/C: (96 h)
TL0 = 300 mg/L
TL50 >1000 mg/L
TL100 >1000 mg/L
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