2-{[4-({4-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]-5-sulfo-1-naphthyl}diazenyl)-(6 or 7)-sulfo-1-naphthyl]diazenyl}benzene-1,4-disulfonic acid, lithium sodium salts

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1987

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

The main structure of the read-across substance is the same of the CJ313, only the salt form is different. However, this difference does not influence the toxicity of the CJ313. CJ313 contains Li/Na salt form and the read-across substances contains only Na salt. This will not lead to difference on the toxicity results.

Qualifier:

according to guideline

Guideline:

other: CIBA Method A-11

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

In principle the methods described in OECD Guideline 203 were used.

GLP compliance:

no

Specific details on test material used for the study:

None

Analytical monitoring:

yes

Details on sampling:

None

Vehicle:

yes

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Rainbow trout: (Salmo gairdneri)
Replicates: 8 per test concentration
Age: about 4-6 months
Size: 4-8 cm
Weight: 3-6 g
Supplier: P. Hohler, 4314 Zeiningen

Golden orfe: (Leuciscus idus)
Replicates: 8 per test concentration
Age: about 12 months
Size: 3-4 cm
Weight: 1.5-2.5 g


Zebrafish: (Danio rerio)
Replicates: 10 per test concentration
Age: about 4-6 months
Size: about 3 cm
Weight: 0.25-0.35 g
Supplier: West Aquarium GmbH, D-3422 Bad Lauterberg

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Remarks on exposure duration:

None

Post exposure observation period:

None

Hardness:

None

Test temperature:

Zebra fish: 23 °C

pH:

7.8

Dissolved oxygen:

None

Salinity:

None

Conductivity:

None

Nominal and measured concentrations:

300 and 1000 mg/L

Details on test conditions:

Water:
15 liters for trout and orfe or 3 liters for zebrafish (active coal filtered). Water used according to ISO guidelines.
Basins:
Full-glass basins, 17 liters basis (36x23x26 cm) tempered (cold-room); 5 liter basins (15x20x20 cm) tempered

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

300 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality occurred at 300 mg/L, while 2 out of 10 fish died at 1000 mg/L concentration.

Results with reference substance (positive control):

None

Reported statistics and error estimates:

None

Validity criteria fulfilled:

not specified

Conclusions:

The LC50 of FAT 40032/D in this fish acute toxicity test was determined to be >1000 mg/L.

Executive summary:

The acute fish toxicity test was performed according to a methodology similar to OECD Guideline 203. Zebrafish: (Danio rerio) were used in the experiment setup. Fish were tested at these different following concentrations: 320 and 1000 mg/L. No mortality occurred at 300 mg/L, while 2 out of 10 fish died at 1000 mg/L concentration.

In conclusion the results obtained were.

LC0 = 300 mg/L

LC50 >1000 mg/L

LC100 >1000 mg/L.

Description of key information

The LC50 of FAT 40032/D in the fish acute toxicity test was determined to be greater than 1000 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 000 mg/L

Additional information

Acute toxicity of Reactive Brown 011 to fish was determined in four available studies. The outcome of all these studies, indicated the LC50 to be >1000 mg/L.

In the key study, Zebrafish: (Danio rerio) were exposed to FAT 40032/D at 300 and 1000 mg/L for 96 hours. No mortality occurred at 300 mg/L while 2 out of 10 fish were found dead at 1000 mg/L concentration.

In conclusion the results obtained were:

LC0 = 300 mg/L

LC50 >1000 mg/L

LC100 >1000 mg/L.

Several other studies are available, results of which are presented below:

FAT 40032/A:

TL0 = 1000 mg/L

TL50 = >1000 mg/L

TL100 = >1000 mg/L

FAT 40032/B: (96 h)

TL0 = 1000 mg/L

TL50 >1000 mg/L

TL100 >1000 mg/L

FAT 40032/C: (96 h)

TL0 = 300 mg/L

TL50 >1000 mg/L

TL100 >1000 mg/L