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EC number: 433-180-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999-03-15 to 1999-03-16
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with acceptable restrictions (Incubation time of 3 hours with permanent aeration instead of 30 minutes as recommended)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- Incubation time of 3 hours with permanent aeration instead of 30 minutes as recommended. Reason: Recommendation of the Umweltbundesamt.
- Principles of method if other than guideline:
- A defined quantity of activated sludge is mixed with synthetic medium and the respiratory rate is measured. This rate is compare to those measured in test preparations with various concentrations of the substance. In order to check the sensitivity of the activated sludge used, 3,5-dichlorophenol is used as a reference substance.
- GLP compliance:
- yes
Test material
- Details on test material:
- -Name of test material (as cited in study report): Reaktiv-Orange DYPR934
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Test concentrations (mg/L) 100, 1000 and 10000
Test substance concentration in physico-chemical consumption and biological oxygen consumption = 10000mg/L (the physico-chemical oxygen consumption is carried out since some substances can also consume oxygen by chemical reactivity. In order to be able to tell the difference between physico chemical oxygen consumption and biological oxygen consumption (respiration), at least the maximum concentration is tested without activated sludge.
Test concentration of the activated sludge: 560 mg/L
- Sample storage conditions before analysis: According to the guideline
- Sampling: Direct weighing (according to SOP 2030-6600401-96 D)
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Incubation time of 3 hours with permanent aeration instead of 30 min incubation time
Invitro/Bac:Biodegradability: Test for Inhibition of oxygen consumption
Bac: defined quantity of activated sludge (mix of different microorganisms, Wupper area water authority) mixed with synthetic medium, respiratory rate is measured
Reference substance 3,5-dichlorophenol
Nominal weights
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Sludge from predominantly domestic sewage
Pretreatment: none
The test substance has been added to about 130 mL deionized water and stirred overnight before testing (equilibration phase)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- With permanent aeration
Test conditions
- Hardness:
- 15.4 mg CaCO3/L
- Test temperature:
- 20±2
- pH:
- 6,8
- Salinity:
- freshwater
- Nominal and measured concentrations:
- Nominal weights
The study is carried out with nominal weights. The test concentrations are not confirmed by chemicals analysis.
100, 1000 and 10000 mg/L
Test concentration of the activated sludge: 360 mg/L
Test substance concentration in physico-chemical oxygen consumption control (mg/L):
10 000 - Details on test conditions:
- A defined quantity of activated sludge is mixed with synthetic medium and the respiratory rate is measured. This rate is compare to those measured in test preparations with various concentrations of the substance. In order to check the sensitivity of the activated sludge used, 3,5-dichlorophenol is used as a reference substance.
- Reference substance (positive control):
- yes
- Remarks:
- 3,5 dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none
- Effect concentrations exceeding solubility of substance in test medium: unknown - Results with reference substance (positive control):
- - Results with reference substance valid yes
EC50 = 13 mg/L
Any other information on results incl. tables
Results
Toxicity to bacteria (test substance) |
Reference substance |
EC 50 > 10000 mg/l |
EC 50 = 13 mg/l |
Measured values of the test substance/control (according to SOP 2030-6600502 -96 D, 2030-0000104-98 D, 2030-0000402 -96, 2030-0000504-98 D)
|
Test concentration [mg/L] |
Initial concentration [mg/250 mL] |
O2-start [mg O2/L] |
O2-end [mg O2/L] |
Time [min] |
Temp [°C] |
pH |
Test Substance |
100 |
25 |
6.4 |
3.7 |
8 |
20.3 |
8.0 |
1000 |
250 |
6.4 |
3.8 |
8 |
20.3 |
8.0 |
|
10000 |
2500 |
5.8 |
3.8 |
7 |
20.4 |
7.9 |
|
Control |
|
|
5.4 |
3.7 |
8 |
20.1 |
7.9 |
Control |
|
|
6.3 |
3.5 |
8 |
20.2 |
8.0 |
Physio-chemical consumption control |
10000 |
2500 |
8.6 |
8.6 |
9 |
20.5 |
7.3 |
Study evaluation
Test concentration [mg/L] |
Respiratory rate test substance [mg/l.h] |
Phys.chem. O2–consumption [mg/l.h] |
Respiratory rate -phys. Chem. - O2–consumption [mg/l.h] |
Inhibition [%] |
100 |
20.3 |
0.0 * |
20.3 |
1.9 |
1000 |
19.5 |
0.0* |
19.5 |
5.8 |
10000 |
17.1 |
0.0 |
17.1 |
17.4 |
|
|
|
|
|
|
|
Comment relevant to result: Test concentrations are given as nominal concentrations and have not been confirmed by analytical methods. *The physico-chemical oxygen consumption has been determined. Therefore lower concentrations of the test substance cause no physico chemical oxygen consumption. |
||
Control, mean |
20.7 |
|||
Control 1 |
20.3 |
|||
Control 2 |
21.0 |
Comments: Because of strong respiration of the activated sludge only 0.56 g/L ss were used.
The test substance has been added to about 130 mL deionized water and stirred overnight before testing (equilibration phase)
Measured values of reference substance
(according to SOP 2030 -6600502 -96 D, 2030-0000104-98 D, 2030-0000402 -96, 2030-0000504 -98 D)
|
Test concentration [mg/L] |
Initial concentration* [mg/250mL] |
O2-start [mgO2/L] |
O2-end [mgO2/L] |
Time [min] |
Temp [°C] |
pH |
Reference substance |
5 |
2.5 |
6.0 |
3.9 |
7 |
20.1 |
7.9 |
10 |
5 |
6.5 |
5.4 |
5 |
20.2 |
8.0 |
|
20 |
10 |
7.8 |
7.1 |
7 |
20.2 |
8.1 |
Comments: *Volume added in mL of 3.5 Dichlorophenol stock solution (500mg/L) total volume 250 mL
Method : 50 % - inhibitory effects:
Test procedure: Respiratory inhibition of activated sludge
organisms according to D. Brown et al., Chemosphere, 10(3),
245-261 (1981 ), and conforming to OECD Guideline 209
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Toxicity to bacteria of Reaktiv Orange DYPR 934 to activated sludge: EC 50 > 10000 mg/L (Reference Substance: EC 50 = 13 mg/L)
- Executive summary:
Toxicity to bacteria of Reaktiv Orange DYPR 934 to activated sludge: EC 50 > 10000 mg/L (Reference Substance: EC 50 = 13 mg/L)
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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