Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): D-Milchsäureisobutylester
- Physical state: fluid
- Analytical purity: 99%
- Impurities: i-Butanol (< 1%)
- Batch No.: Cont. TTTU 119 618

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Stock solution: Tets substance was directly added. Nominal concentration of the stock solution = 100 mg/L

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: Originally from "Institut National de Recherche Chimique appliquee, France", and has been bred from 1978 in the Laboratories of BASF
- Age at study initiation: 2 - 24 h
- Feeding during test: none

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.44 mmol/L
Test temperature:
19.6 - 20.6 °C
pH:
7.7 - 8.0
Dissolved oxygen:
7.4 - 8.4 mg/L
Nominal and measured concentrations:
Nominal test concentrations: control, 6.25, 12.5, 25, 50, 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, nominal volume, test volume: glass tubes with flat bottom, 20 ml, 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic fresh water
- Alkalinity (pH 4.3): 0.9 ± 0.5 mmol/L
- Ca/Mg ratio: ca. 4:1
- Conductivity: 600 - 700 µS/cm
- Intervals of water quality measurement: temperature after 0, 24, 48 h in seperate test vessels, pH and oxygen content in one replicate of each test concentration

OTHER TEST CONDITIONS
- Photoperiod: day : night interval 16 : 8 h
- Light intensity: ca. 5-6 µE/m²s, wavelength 400 - 700 nm

EFFECT PARAMETERS MEASURED: mobility after 0, 3, 6, 24, 48 hours

Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion