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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-methylpropyl-(R)-2-hydroxypropanoate
EC Number:
407-770-0
EC Name:
2-methylpropyl-(R)-2-hydroxypropanoate
Cas Number:
61597-96-4
Molecular formula:
C7 H14 O3
IUPAC Name:
2-methylpropyl-(R)-2-hydroxypropanoate
Details on test material:
- Name of test material (as cited in study report): D-Milchsäureisobutylester
- Physical state: fluid
- Analytical purity: 99%
- Batch No.: Cont. TTTU 119 618

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
sewage, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge: Effluent of a laboratory wastewater plant treating municipal sewage
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
20 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
SAMPLING
- Sampling frequency: after 0, 1, 4, 6, 10, 14, 17, 20, 24, 27, 28 days

CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 replicates
- Reference substance: 1 replicate
- Toxicity control: 1 replicate



Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

% Degradation
Parameter:
% degradation (DOC removal)
Value:
96
Sampling time:
28 d
Details on results:
- Kinetic of the degradation process:
after day 1: 7.4%
after day 4: 46.5%
after day 6: 76.6%
after day 20: 95.3%

BOD5 / COD results

Results with reference substance:
- Degradation of reference substance: 99% after 28 days

Applicant's summary and conclusion

Interpretation of results:
readily biodegradable