Diethyl [[3,5-bis(1,1-dimethylethyl)-4-hydroxyphenyl]methyl]phosphonate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1967

Report date:

1967

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Diethyl [[3,5-bis(1,1-dimethylethyl)-4-hydroxyphenyl]methyl]phosphonate

EC Number:

213-551-9

EC Name:

Diethyl [[3,5-bis(1,1-dimethylethyl)-4-hydroxyphenyl]methyl]phosphonate

Cas Number:

976-56-7

Molecular formula:

C19H33O4P

IUPAC Name:

diethyl (3,5-di-tert-butyl-4-hydroxybenzyl)phosphonate

Test animals

Species:

rabbit

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: young adult animals
- Weight at study initiation: average body weight of about 3 kg
- Housing: single housing
- Diet: standard rabbit food ad libitum
- Water: ad libitum
- Acclimation period: 7 days

Administration / exposure

Type of coverage:

occlusive

Vehicle:

water

Remarks:

50% aqueous suspension

Details on dermal exposure:

TEST SITE
- Area of exposure: about 10 % of the total body surface area
- Type of wrap if used: impervious plastic sheeting
- Preparation of test site: The backs of the rabbits were shaved free of hair with electric clippers, the shaved area constituting about 10 % of the total body surface-area. Additionally, epidermal abrasions were made every 2 or 3 centimeters longitudinally over the area of exposure on one female and one male animal.

REMOVAL OF TEST SUBSTANCE
- Washing: yes
- Time after start of exposure: after removing of the wrap

TEST MATERIAL
- Amount applied: 5 g/ kg bw
- Concentration: 50 % (w/v) aqueous suspension
- Constant volume or concentration used: yes

Duration of exposure:

24 hours

Doses:

5000 mg/kg body weight

No. of animals per sex per dose:

2

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: weighing: before application, on day 8 and on day 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, histopathology

Statistics:

not required

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Mortality:

none

Clinical signs:

other: none

Gross pathology:

no findings

Any other information on results incl. tables

Scarified and unscarified rabbits gave indistinguishable results.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met