Benzyl cinnamate

Basic toxicokinetics

Administrative data

Endpoint:

basic toxicokinetics in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1977

Materials and methods

Objective of study:

metabolism

other: enzymatic hydrolysis

Principles of method if other than guideline:

In vitro hydrolysis procedure using preparation of pancreatin.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Benzyl cinnamate

EC Number:

203-109-3

EC Name:

Benzyl cinnamate

Cas Number:

103-41-3

Molecular formula:

C16H14O2

IUPAC Name:

benzyl 3-phenylacrylate

Test animals

Species:

other: origin of the pancreatin preparation is unclear

Strain:

not specified

Administration / exposure

Route of administration:

other: not applicable as it is an in vitro study

Details on study design:

The test material was incubated in 0.5 M phosphate buffer at pH 7.5 and 37° C with preparation of pancreatin (Merck 7132). The extent of hydrolysis was determined after 2 h by gas--liquid chromatography of the ester.

Results and discussion

Main ADME results

Type:

other: % hydrolysis

Results:

80

Metabolite characterisation studies

Metabolites identified:

no

Any other information on results incl. tables

The author showed that the substance hydrolyses rapidly in vitro and presumed that because of this the ester will also be degraded readily in the intact animal.

 

Ester	Conc (µL/L) ^a	GLC column used for		% Hydrolysis
		Ester	Alcohol
Test item	18 mg/L ^e	Dexsil 300	Carb 20 M	80

^a concentration in incubation mixture in µL/L unless otherwise stated

^e esters dissolved in ethanol initially and then diluted with water

Applicant's summary and conclusion

Conclusions:

The ester was hydrolysed rapidly by pancreatin in vitro.

Executive summary:

The study is a preliminary investigation using an in vitro hydrolysis procedure designed to establish the relative rates of enzymic hydrolysis of the ester. The test substance was incubated with a preparation of pancreatin (Merck 7132) in 0.5 M phosphate buffer at pH 7.5 and 37° C. Incubations were made under arbitrary experimental conditions and the extent of hydrolysis of the ester by pancreatin was determined after 2 h by gas-liquid chromatography. The test substance showed 80% hydrolysis under the conditions of the experiment, indicating that the ester hydrolyses rapidly by pancreatin.

The author presumes that esters hydrolysing rapidly in vitro, will also be degraded rapidly in the intact animal.