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Reaction mass of Cobaltate(1-), bis[6-(amino-κN)-5-[2-[2-(hydroxy-κO)-4-nitrophenyl]diazenyl-κN1]-N-methyl-2-naphthalenesulfonamidato(2-)]-, sodium (1:1) and disodium [6-amino-5-[(2-hydroxy-4-nitrophenyl)azo]-N-methylnaphthalene-2-sulphonamidato(2-)][6-amino-5-[(2-hydroxy-4-nitrophenyl)azo]naphthalene-2-sulphonato(3-)]cobaltate(2-)
EC number: 943-062-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- Preparation of the inoculum: Higher sludge weight after the washing procedure (Sludge concentrations in the test according to the guidelines) and 4 instead of 5 test concentrations.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Code number: FAT 20036/F
Batch-No: 1
Purity: ca. 97.7%
Appearance: Solid
Solubility: 80 mg/L (in water)
Storage: room temperature
Expiration date: 07/99 - Analytical monitoring:
- no
- Details on sampling:
- Not available
- Vehicle:
- no
- Details on test solutions:
- Not available
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Organism: Activated sludge from a communal sewage treatment plant (ARA -Pro Rheno Basel)
- Sludge pretreatment: 3 times washing with tap water and addition of synthetic sewage (a day before the main test). - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- None
- Post exposure observation period:
- Not available
- Hardness:
- Not available
- Test temperature:
- 20.8 °C
- pH:
- 7.3 before the test beginning
- Dissolved oxygen:
- measured
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- - Stock solutions: 500 mg/l test substance / reference substance in deionized water.
- Test concentrations : 320, 100, 32 and 10 mg/l of the test substance and 32, 10 and 3.2 mg/l of the reference substance - Details on test conditions:
- - Temperature : 20.8 °C
- Sludge dry weight: 1.50 g/l
- Test volume : 100 ml
- Test duration: 3 h
- Estimation technique : O2 - consumption, measured with an oxygen sensitive electrode, system : OXI DIGI 530 (SAV : 00 109)
- Aeration : 0.1 - 0.2 l/min by pasteur-pipette.
- pH of sludge : 7.3 before the test beginning - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol (Merck 821768)
- Key result
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- Not available
- Results with reference substance (positive control):
- The IC50 (3 h) of the reference substance (3,5-Dichlorophenol) was found to be 9.0 mg/l
- Reported statistics and error estimates:
- Not available
- Validity criteria fulfilled:
- yes
- Conclusions:
- The IC50 (3 h) of the test substance FAT 20036/F was found to be greater than 320 mg/l
- Executive summary:
The toxicity of FAT 20036/F to activated sludge was determined in a 3-hours respiration inhibition test according to the EEC Method No. L 133/118 -122 and to the OECD Guideline No. 209. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland.
The test substance concentrations tested were 320, 100, 32 and 10 mg/l. The reference substance, 3,5-Dichlorophenol was tested in concentrations of 32, 10 and 3.2 mg/l.
The IC50 (3 h) of the test substance FAT 20036/F was found to be greater than 320.0 mg/l
IC50 (3 h) of the reference substance 3,5-Dichlorophenol was found to be 9.0 mg/l
Reference
ACTIVATED - SLUDGE - INHIBITION / IC50 (3 h)
OXYGEN - CONSUMPTION - TEST
Control | pH after 3 hrs | 1) O2 -consumption (in cm) | 2) Time (in cm) | mg O2/min/L | C=control1) 2 cm = 0.8 mg/l O22) 2 cm = 1 min |
C1 | 8.0 | 3.7 | 4.0 | 0.74 | C1/C2: 0.00% C2/C1: 0.00% |
C2 | 7.8 | 3.5 | 3.8 | 0.74 | Mean C: 0.74 |
Test substance mg/L | pH after 3 hour | 1) O2 -consumption (in cm) | 2) Time (in cm) | mg O2 pro min/L | % of control | % inhibition |
320 | 7.6 | 4.0 | 4.7 | 0.68 | 91.89 | 8.11 |
100 | 7.8 | 3.8 | 4.4 | 0.69 | 93.24 | 6.76 |
32 | 7.9 | 4.0 | 4.4 | 0.73 | 98.65 | 1.35 |
10 | 7.9 | 3.8 | 4.4 | 0.69 | 93.24 | 6.76 |
3,5 -Dichlrophenoö (mg/L) | pH after 3 hour | 1) O2 -consumption (in cm) | 2) Time (in cm) | mg O2 pro min/L | % of control | % inhibition |
32 | 8.0 | 0.7 | 4.7 | 0.12 | 16.22 | 83.78 |
10 | 7.9 | 1.9 | 4.3 | 0.35 | 47.30 | 52.70 |
3.2 | 7.9 | 3.0 | 4.2 | 0.57 | 77.03 | 22.97 |
Test substance IC50 (3 h) = > 320.0 mg/l
3,5-Dichlorophenol IC50 ( 3 h) = 9.0 mg/l
Description of key information
A key study was performed to determine the toxicity of FAT 20036/F to activated sludge was determined in a 3-hours respiration inhibition test according to the EEC Method No. L 133/118 -122 and to the OECD Guideline No. 209. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland.
The IC50 (3 h) of the test substance FAT 20036/F was found to be greater than 320.0 mg/l
IC50 (3 h) of the reference substance 3,5-Dichlorophenol was found to be 9.0 mg/l.
Several studies, (supporting studies), were also performed on the different batches to test the toxicity on micro-organisms.
BST, (toxicity to micro-organisms), was found to be greater than 300 mg/L on batch A, B, C and D.
For the batch F, IC50 was found to be greater than 320 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 320 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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