(4-carboxybutyl)triphenylphosphonium bromide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2020

Report date:

2020

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

(4-carboxybutyl)triphenylphosphonium bromide

EC Number:

241-782-5

EC Name:

(4-carboxybutyl)triphenylphosphonium bromide

Cas Number:

17814-85-6

Molecular formula:

C23H24BrO2P

IUPAC Name:

(4-carboxybutyl)triphenylphosphonium bromide

Test material form:

solid

Details on test material:

Purity (certified) 100.7%

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUS, obtained from continuous laboratory
cultures
- Age at study initiation (mean and range, SD): Less than 24 hours old daphnids from a healthy stock
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu),14195 Berlin, Germany
- Feeding during test : No

ACCLIMATION
Acclimatisation of the daphnids was not necessary, because the dilution water was equivalent to the culture medium

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

267 mg CaCO3/L (Water Quality Parameters of the Dilution Water at the Start of the Exposure)

Test temperature:

19 - 20 °C.

pH:

7.21 - 7.75

Dissolved oxygen:

7.93 - 8.88

Conductivity:

585 μS/cm (Water Quality Parameters of the Dilution Water at the Start of the Exposure)

Nominal and measured concentrations:

nominal 100 mg/L
Fresh medium (0 hours) 103 mg/L
Old medium (48 hours) 101 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beakers (4 (ID) x 7 (H) cm), 50 mL capacity, loosely covered with watch glasses
- Volume of solution: 20 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8 hours light/dark cycle
- Light intensity: Diffuse light, light intensity of max. 1500 lx

RANGE-FINDING STUDY
- Test concentrations: 100, 10, 1 mg/l
- Results used to determine the conditions for the definitive study: yes

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

The measured concentration of the test item in fresh media at the start of the exposure (0 hours) was 103% of the nominal concentration. At the end of the exposure (48 hours), the measured test item

concentration was 101% of the nominal concentration.

In the limit concentration 100 mg/L of the test item (4-carboxybutyl)triphenylphosphonium bromide (CBT), no effects (no immobilzed daphnids) on Daphnia magna were observed.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In the limit concentration 100 mg/L of the test item (4-carboxybutyl)triphenylphosphonium bromide (CBT), no effects on Daphnia magna were observed.