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EC number: 287-828-8 | CAS number: 85586-25-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Sediment toxicity
Administrative data
- Endpoint:
- sediment toxicity: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OSP ARCOM guidelines adopted in 1995
- GLP compliance:
- yes
Test material
- Reference substance name:
- Fatty acids, rape-oil, Me esters
- EC Number:
- 287-828-8
- EC Name:
- Fatty acids, rape-oil, Me esters
- Cas Number:
- 85586-25-0
- Molecular formula:
- Unspecified
- IUPAC Name:
- methyl (11Z)-icos-11-enoate; methyl (13Z)-docos-13-enoate; methyl (9E)-octadec-9-enoate; methyl (9Z,12Z)-octadeca-9,12-dienoate
- Details on test material:
- - Name of test material (as cited in study report): rapeseed oil fatty acid methyl ester
- Physical state: yellowish liquid
- Analytical purity: 100% AM
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- no
- Details on sediment and application:
- Sediment for use in toxicity testing is collected from a point adjacent to the site of Corophium collection. On return to the Iaboratory, the sediment is wet-sieved through 0.6 mm mesh to remove larger infaunal organisms and Corophium within the test size range, and allowed to settle in tall polyethylene containers. After settling, the supernatant water is decanted and the sediment stored in a room at approximately 4 °C,
until required for testing. Immediately before the initiation of the test, the sediment is thoroughly homogenised and a representative sample taken for wet and dry weight determination. The ratio of wet weight to dry weight is subsequently used to convert nominal exposure concentrations on a wet weight basis to nominal exposure concentrations on a dry weight basis.
Test organisms
- Test organisms (species):
- Corophium volutator
- Details on test organisms:
- Organisms are collected from a site in the Bay of Suckquoy, near Sebay farm in Orkney. Specimens are gently sieved from their native sediment and held in plastic containers (approximately 51 capacity) containing some ambient water, with a small amount of detritus, until transfer to the laboratory. On return to the laboratory, the Corophium are transferred in ambient water to polythene tanks of approximately 200 litre capacity, gentle aeration is supplied. The tanks are located in a controlled-temperature room at approximately 15 °C. The stock animals are gradually acclimated from the ambient salinity of less than 5‰ tothat of undiluted seawater (approximately 35‰) in increments of approximately 10‰ per day. Once acclimation is complete, the stock tanks are maintained under semi-static conditions until the initiation of the test. The holding period in the Iabaratory is between 3 and 14 days. Sufficient detrital material is retained in each tank to provide food and some bottarn cover, but not of a density that prevents daily observation of mortality and morbidity. Dead or impaired animals are removed when observed. Specimens of approximately 5 mm in body length (excluding rostrum) are used in the toxicity tests.
Study design
- Study type:
- laboratory study
- Test type:
- static
- Water media type:
- saltwater
- Type of sediment:
- natural sediment
- Limit test:
- no
Exposure duration
- Duration:
- 10 d
- Exposure phase:
- total exposure duration
Test conditions
- Test temperature:
- 14.7-16.4 °C
- pH:
- 7.91 - 8.35
- Dissolved oxygen:
- 92 - 98% saturation
- Salinity:
- 35 ‰
- Nominal and measured concentrations:
- Nominal: 10, 100, 320, 1000 and 10000 mg/kg ww
Nominal: 17.38, 174.09, 552.87, 1707.82 and 16011.13 mg/kg dw - Details on test conditions:
- Tests are conducted in 1 litre capacity glass beakers each containing 2 cm depth (approximately 150 ml) of amended sediment and 850 ml of overlying seawater (1 µm filtered ultra violet treated seawater). Test beakers are maintained under constant dim illumination in a controlled temperature room for a test period of 10 days. Three replicates are prepared for each test concentration; controls are replicated five times. The beakers are assigned positions within the test area, arranged in rows of three to five and spaced to maintain effective separation of different treatments. Each row is covered with a reetangular sheet of perspex perforated with a small hole above the centre of each beaker. Aeration is provided and a stream of air bubbles are released at a depth of approximately 6 cm. Twenty animals are added to a 100 rml beaker and the contents are then dispensed into a test vessel, one beaker per vessel. Sixty organisms are exposed in total per concentration and one hundred in five vessels for controls. The finallevel of water in each test vessel is marked with a permanent marker. At the end of the test period, the contents of each beaker are gently sieved through a 0.6 mm mesh, and all surviving animals separated and counted. Missing animals are counted as dead; early mortalities will either have been consumed by survivors or have decomposed during the test period.
- Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 10 d
- Dose descriptor:
- LC50
- Effect conc.:
- 3 367.41 mg/kg sediment dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance exhibited a 10d-LC50 value of 3367.41 mg/kg dw to the marine amphipod Corophium volutator in the sediment phase.
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