3-methylbutyl isovalerate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999-05-18 to 1999-07-22

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

1992

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: analysis of all test concentrations and a control at test start and after 48 h of exposure

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 79 mg/L of the substance was weighed into water, treated for 60 seconds at 8000 rpm. with an ultra-turrax and afterwards stirred for 24 hours on a magnetic stirrer. Emulsion was filtered using a folded filter of the pore size 7-12 µm to eleminate undissolved particles. M4-Medium according to Elendt and BGA (1992) used for dilution.
- Controls: yes; M4-Medium according to Elendt and BGA (1992)

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Source: cloned at the Federal Health Office (BGA) in Berlin; Origin of test animals: laboratory bred
- Age at study initiation (mean and range, SD): 0 - 24 h

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

15.3°dH (dilution water)

Test temperature:

19.4 - 19.7°C (48 h)

pH:

7.8 - 8.1 (48 h)

Dissolved oxygen:

7.4 - 8.4 mg/L (48 h)

Nominal and measured concentrations:

- Nominal concentration: 0 (control), 3.2, 6.3, 12.6 mg test substance/L
- Measured concentration: 0 h:
- According to the relevant product information, 1 mg/l TOC equals to 1.4 mg/l of the test substance (structural formula: C10H2002; molecular weight: 172.3 g/mol).

Details on test conditions:

TEST SYSTEM
- Test vessel: so called "Karlsruher bottles" with stoppers
- Type: closed
- Material, size, headspace, fill volume: 250 - 300 mL fill volume and had space
- Aeration: not ventilated
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4-Medium according to Elendt and BGA (1992)
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light / 8 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- number of immobile daphnids after 24 h and 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study
- Results used to determine the conditions for the definitive study: considering the decrease of the test concentration during the preliminary experiment, the main test was conducted under closed-bottle conditions in so-called ,Karlsruher Flaschen'.

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

3.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 12 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

3.5 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

10.5 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

6.1 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Results after 24 h and 48 h

Nominal concentration [mg/l]	Results after 24 h and 48 h
	Immobile daphnids				Oxygen content [mg/L] after 48 h	pH after 48 h	Temperature [°C] after 48 h
	absolute		%
	24 h	48 h	24 h	48 h
0 (control)	0	0	0	0	8.4	8.1	19.7
3.2	0	0	0	0	8.1	8.0	19.5
6.3	6	10	30	50	7.7	7.9	19.4
12.6	12	20	60	100	7.4	7.8	19.5

Validity criteria fulfilled:

yes

Conclusions:

The toxicity of the test item on Daphnia magna was determined in a GLP study according to OECD 202. The 48-h EC50 was calculated as 6.1 mg/L (geom. mean (EC0/EC100) of arithmetic mean of analytical values).

Description of key information

The toxicity of the test item on Daphnia magna was determined in a GLP study according to OECD 202. The 48-h EC50 was 6.1 mg/L (geom. mean (EC0/EC100) of arithmetic mean of analytical values).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

6.1 mg/L

Additional information

The toxicity of test item on Daphnia magna was determined in a GLP study according to OECD 202. Individuals of D. magna were exposed to 0 (control), 3.2, 6.3, 12.6 mg/L of the test substance, equivalent to geometric mean measured concentrations of <LOQ, 2.8, 5.6, 9.8 mg/L after 24h and <LOQ, 4.2, 7.0, 11.2 mg/L after 48h. Concentrations were analytical verified via TOC determination. 100% immobility of D. magna was observed after 48h at 10.5 mg/L. No mortality was observed after 48h at 3.5 mg/L. The 48h EC50 value of 6.1 mg/L was calculated as geometric mean of EC0 and EC100.