Registration Dossier
Registration Dossier
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EC number: 304-661-9 | CAS number: 94277-77-7
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
The 96-h LC50 value is >100 mg/L (loading rate) in fish (D. rerio).
Key value for chemical safety assessment
Additional information
The short-term toxicity to freshwater fish was determined in a study according to OECD guideline No 203 and in compliance with GLP criteria (Dr. Noack-Laboratories, 2015).
The test was performed as limit test using the saturated solution of the substance as single concentration. Groups of 7 zebrafish (D. rerio) were exposed to nominal test concentrations of 0 (control) and 100 mg/L for 96 hours under semi-static conditions (daily renewal). Test concentrations were not analytically verified because the water solubility limit was below the analytical limit of quantification. Observations for mortalities and symptoms of toxicity were made at 2, 24, 48, 72 and 96 hours after the start of exposure. Throughout the test no mortality or symptoms of toxicity were observed. Based on these findings the 96 -h LC50 value was determined at >100 mg/L i.e. higher than the solubility limit of the test substance in water.
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