2-[{6-[(4-amino-6-chloro-1,3,5-triazin-2-yl)(methyl)amino]-1-hydroxy-3-sulfonaphthalen-2-yl}diazenyl]naphthalene-1,5-disulfonic acid, lithium sodium salts

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From February 8, 2017 to March 10, 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test organisms (species):

Oryzias latipes

Details on test organisms:

- Source: National Chiayi University Freshwater Biological Resource Center
- Length at study initiation: 2.0 ± 1.0 cm

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Nominal and measured concentrations:

For the range-finding test, the nominal concentrations were 0.08, 0.16, 4.00, 20.00 and 100.00 mg/L.
For the limit test, the nominal concentration was 100.00 mg/L.

Details on test conditions:

- Test volume: 2 L
- Photoperiod: 12-16 hours daily

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Table 1. Mortality and LC50 in the range-finding test

Group (mg/L)^a		Mortality (%)^b				LC50 (mg/L)
		24 ± 1 hours	48 ± 2 hours	72 ± 3 hours	96 ± 3 hours
Control group	0.00	0.00	0.00	0.00	0.00	−
Test group	100.00	0.00	0.00	0.00	0.00	> 100.00
	20.00	0.00	0.00	0.00	0.00
	4.00	0.00	0.00	0.00	0.00
	0.16	0.00	0.00	0.00	0.00
	0.08	0.00	0.00	0.00	0.00

^a All one replicate was conducted at the control group and test groups in range-find test.

^b Mortality (%) = Death number / Total number × 100%

Table 2. Temperature, dissolved oxygen and pH value in the range-finding test

Group (mg/L)^a		Time (hour)	Temperature (°C)	Dissolved oxygen (%)	pH value
Control group	0.00	0	22.0	99.0	6.58
		24 ± 1	23.0	88.5	7.70
		48 ± 2	23.0	86.2	7.65
		72 ± 3	22.0	85.0	7.78
		96 ± 3	21.5	86.2	6.00
Test group	100.00	0	22.0	98.7	6.75
		24 ± 1	23.0	90.2	7.60
		48 ± 2	23.0	88.2	6.97
		72 ± 3	22.0	86.0	7.96
		96 ± 3	21.5	84.4	6.13

^a One replicate was only conducted at the control group and highest concentration test group (100.00 mg/L) in the range-find test.

 

Table 3. Mortality in the limit test

Group (mg/L)^a		Mortality (%)^b
		24 ± 1 hours	48 ± 2 hours	72 ± 3 hours	96 ± 3 hours
Control group	0.00	0.00	0.00	0.00	0.00
Test group	100.00	0.00	0.00	0.00	0.00

^a All one replicate was conducted at the control group and test groups in the limit test.

^b Mortality (%) = Death number / Total number × 100%

 

Table 4. Temperature, dissolved oxygen and pH value in the limit test

Group (mg/L)^a		Time (hour)	Temperature (°C)	Dissolved oxygen (%)	pH value
Control group	0.00	0	21.5	99.9	6.59
		24 ± 1	22.0	86.0	6.35
		48 ± 2	22.0	79.4	6.21
		72 ± 3	21.0	78.6	6.67
		96 ± 3	21.0	74.4	7.06
Test group	100.00	0	21.5	99.8	6.78
		24 ± 1	22.0	83.7	6.14
		48 ± 2	22.0	85.3	6.32
		72 ± 3	21.0	84.6	6.61
		96 ± 3	21.0	76.9	6.82

^a All one replicate was only conducted at the control group and test group in the limit test.

Validity criteria fulfilled:

yes

Conclusions:

According to OECD 203 test method, the 96-hr LC50 of CJ302 was greater than 100.00 mg/L.

Executive summary:

This test using the procedures outlined in the SuperLab Study Plan for M62-161200028001EN which is based on the SOP for the OECD203 (SOPE-509) and OECD203 (OECD, 1992). All test validity criteria were met. At the range-finding test, the mortality of 0.08-100.00 mg/L were all 0.00% and 96-hr LC50 was greater than 100.00 mg/L. Base on this result of range-finding test, the highest concentration of range-finding test (100.00 mg/L) was used to conduct the limit test. Therefore, the limit test was conducted and results showed that the mortality of 100.00 mg/L was 0.00% at the end of test. Under the conditions of this test, the 96-hr LC50 of CJ302 was greater than 100 mg/L.

Description of key information

The 96-hr LC50 of CJ302 was greater than100 mg/L for ricefish (Oryzias latipes) (OECD TG203).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

The mortalities of control group were smaller than 10% in both range-finding and limit test. The temperature deviation were within ± 2 °C and dissolved oxygen were ≥ 60 %.All test validity criteria were met. At the range-finding test, the mortality of 0.08-100.00 mg/L were all 0.00% and 96-hr LC50 was greater than 100.00 mg/L. Base on this result of range-finding test, the highest concentration of range-finding test (100.00 mg/L) was used to conduct the limit test. Therefore, the limit test was conducted and results showed that the mortality of 100.00 mg/L was 0.00% at the end of test. Under the conditions of this test, the 96-hr LC50 of CJ302 was greater than 100 mg/L.