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Diss Factsheets
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EC number: 201-942-7 | CAS number: 89-81-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- The study was conducted in 1980.
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Study is well conducted but its design is not sufficient to cover the endpoint.
Data source
Reference
- Reference Type:
- publication
- Title:
- SCREENING OF TOBACCO SMOKE CONSTITUENTS FOR MUTAGENICITY USING THE AMES' TEST.
- Author:
- Florin I et al.
- Year:
- 1 980
- Bibliographic source:
- Toxicology 18 (1980) 219 – 232.
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Deviations:
- not applicable
- Principles of method if other than guideline:
- Substances were assayed for mutagenicity towards four histidine-requiring mutants of Salmonella typhimurium (TA 98, TA 100, TA1535 and TA 1537). The test was performed with and without metabolic activation using a liver fraction (S-9) from Aroclor 1254 of methylchloanthrene induced rats using a modified Ames test. Due to the large number of compounds screened during the study potential mutagens were screened using spot tests which are less sensitive than quantitative experiments.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 6-isopropyl-3-methylcyclohex-2-enone
- EC Number:
- 201-942-7
- EC Name:
- 6-isopropyl-3-methylcyclohex-2-enone
- Cas Number:
- 89-81-6
- Molecular formula:
- C10H16O
- IUPAC Name:
- 6-isopropyl-3-methylcyclohex-2-enone
- Test material form:
- not specified
- Details on test material:
- Test substance: Piperitone
Purity: > 97 %
Constituent 1
Method
- Target gene:
- Histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- 3 µmol/plate
- Vehicle / solvent:
- Ethanol.
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- no
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- N-ethyl-N-nitro-N-nitrosoguanidine
- other: 2-aminoanthracene
- Details on test system and experimental conditions:
- Revertants were scored on glucosen minimal salts medium supplemented with 0.05 µmol histidine and 0.05 µmol biotin. Plates used for viable counts contained 10 µmol histidine (and 0.05 µmol biotin).
The following controls were made for each experiment
The viable count was determined.
The number of spontaneous revertants was measured.
The presence of the rfa-mutation was checked by crystal violet inhibition.
The presence of the plasmid pKM 101 in strains TA98 and TA100 was checked by resistance to ampicillin.
The response of the positive controls, N-methyl-N'-nitro-N-nitrosoguanidin (not requiring metabolic activation) and 2-aminoanthracene (requiring activation) was checked.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- not examined
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Additional information on results:
- The test substance was not found to be mutagenic.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The test substance was not found to be mutagenic. - Executive summary:
The genotixic potential of the test substance was assessed using a modified Ames test in which potential mutagens were screened using spot tests. The test substance was not found to be mutagenic under the conditions of the test, both with and without metabolic activation.
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