Registration Dossier
Registration Dossier
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EC number: 205-275-2 | CAS number: 137-05-3
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-study
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2002
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Methyl cyanoacetate
- EC Number:
- 203-288-8
- EC Name:
- Methyl cyanoacetate
- Cas Number:
- 105-34-0
- Molecular formula:
- C4H5NO2
- IUPAC Name:
- methyl cyanoacetate
- Details on test material:
- Analogy; data taken from CAS 105-34-0 methyl cyanoacetate
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Suspended matter: 23.58 mg/L
- Dry matter: 3.93 mg/L - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 17.2 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Test temperature: 22.1°C
- pH: 7.4
TEST SYSTEM
- Culturing apparatus: 2000 mL conical flasks with loose aluminium foil closure
- 2 flasks containing the test item (17.22 mg DOC/l after 3 h) and inoculum
- 2 flasks without test item, but with inoculum (=blank batches)
- 2 flasks containing the reference item sodium benzoate (15.98 mg DOC/L after 3h) and inoculum (= reference batches)
SAMPLING
- Sampling frequency: 0 and 3 hours, 7, 14, 21, 27 and 28 days
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (DOC removal)
- Value:
- 0
- Sampling time:
- 0 h
- Parameter:
- % degradation (DOC removal)
- Value:
- 94
- Sampling time:
- 7 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 96
- Sampling time:
- 14 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 97
- Sampling time:
- 21 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 99
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- Kinetic of control substance (sodium benzoate):
0% after 0 hours, 100% after 7 days, 99% after 14 days, 99% after 21 days, 99% after 28 days
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Under present conditions the biodegradability of the test item was 99% of the initial amount after 28 days.
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