Hydroxylamine

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Unsuitable test system, as the inhalation hazard test is insufficient for non-volatile substances.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1969

Report date:

1969

Materials and methods

Principles of method if other than guideline:

The inhalation hazard test demonstrates the toxicity of an atmosphere saturated with vapors of the volatile components of a test substance at the temperature chosen for vapor generation (usually 20°C). Several groups of usually 3 rats per sex were sequentially exposed to the vapors, generated by bubbling 200 l/h air through a substance column of about 5 cm above a fritted disc in a glass cylinder for different time periods.
No analytical determination of the atmosphere concentrations was performed. The nominal concentration usually can be calculated as quotient of the amount of the test substance weight loss during exposure. Group-wise documentation of clinical signs was performed over the 14 day study period. The study was conducted acc. to the method decribed by Smyth et al. 1962 with acceptable modifications.

GLP compliance:

no

Remarks:

; GLP was not compulsory at the time the study was conducted.

Test type:

other: Inhalation Hazard Test

Test material

Test material information

Constituent 1

Reference substance name:

Bis(hydroxylammonium) sulphate

EC Number:

233-118-8

EC Name:

Bis(hydroxylammonium) sulphate

Cas Number:

10039-54-0

IUPAC Name:

bis(hydroxyammonium) sulfate

Details on test material:

- Name of test material (as cited in study report): Hydroxylammoniumsulfat
- Physical state: solid (vapour pressure is negligible at 20 °C)
- Analytical purity: pure grade

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Average weight at study initiation: experiment 1: ca. 216 g; experiment 2: ca. 218 g

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Details on inhalation exposure:

Saturation of the atmosphere was generated by conducting an air stream through a substance layer of 5 cm height at 20 °C.
The rats were exposed to the test atmosphere for 8 hours.
Air flow was 200 l/h in the exposure chamber.

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

8 h

Concentrations:

The concentration was 0.0 mg/l (20 °C), no volatile parts of the test material were generated; the nominal concentration was calculated as quotient of the amount of the test substance weight loss during exposure.

No. of animals per sex per dose:

3 animals per sex per group

Control animals:

no

Details on study design:

The exposure time not causing lethality was tested twice.
- Duration of post exposure period until necropsy: 4 days
- Frequency of weighing: Test animals were weighed at test initiation and before sacrifice
- Frequency of observations: The animals were observed following exposure after 3 min, 10 min, 30 min, 1 hour, 3 hours, 7 hours and thereafter daily
- Necropsy of survivors performed: yes. The necropsy of the animals was done on day 4 following exposure

Results and discussion

Mortality:

All 12 animals survived after 8 h, and no deaths occurred in the postexposure period.

Clinical signs:

other: No symptoms were noted.

Body weight:

Mean body weight at the end of the study:
- IHT room temperature, 8 hours, experiment 1: 220.2 g
- IHT room temperature, 8 hours, experiment 2: 233 g

Gross pathology:

Autopsy revealed no relevant findings.

Other findings:

No formation of dust was seen.