Reaction mass of 2-methylbutyl salicylate and pentyl salicylate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

15 Sept 1996 to 13 Nov 1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted in accordance with relevant OECD guideline and to GLP standards.

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

GLP compliance:

yes

Specific details on test material used for the study:

- Name of test material (as cited in study report): AMYL SALICYLATE
- Molecular formula (if other than submission substance): C12H16O3
- Molecular weight (if other than submission substance):208.3 g/mol
- Structural formula attached as image file (if other than submission substance): see Fig.
- Substance type: Mixture of two isomers (ratio not reported)
- Physical state: Liquid
- Analytical purity: 98.5% (sum of two isomers)
- Lot/batch No.: 261891

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Duration of test (contact time):

28 d

Initial conc.:

100.4 mg/L

Based on:

test mat.

Initial conc.:

101.5 mg/L

Based on:

test mat.

Initial conc.:

102.6 mg/L

Based on:

test mat.

Initial conc.:

101.7 mg/L

Based on:

test mat.

Parameter:

% degradation (O2 consumption)

Remarks:

BOD, mgO2/L adjusted to nominal concentrations

Value:

32

Sampling time:

2 d

Remarks on result:

other: Mean of two tests

Parameter:

% degradation (O2 consumption)

Remarks:

BOD, mgO2/L adjusted to nominal concentrations

Value:

63

Sampling time:

7 d

Remarks on result:

other: Mean of two tests

Parameter:

% degradation (O2 consumption)

Remarks:

BOD, mgO2/L adjusted to nominal concentrations

Value:

80

Sampling time:

12 d

Remarks on result:

other: Mean of two tests

Parameter:

% degradation (O2 consumption)

Remarks:

BOD, mgO2/L adjusted to nominal concentrations

Value:

83

Sampling time:

14 d

Remarks on result:

other: Mean of two tests

Parameter:

% degradation (O2 consumption)

Remarks:

BOD, mgO2/L adjusted to nominal concentrations

Value:

86

Sampling time:

28 d

Remarks on result:

other: Mean of two tests

Reference material: Aniline (99.5% min.)

 

Toxicity control:

The toxicity of the test substance to the innoculum was checked by measuring the inhibitory rate on respiration compared to a control sample with test substance. The toxicity control and control contained the following:

Toxicity control: Mineral medium + test substance (100mg/L) + aniline (100mg/L, nominal) + innoculum

Control: Mineral medium + aniline (100mg/L, nominal) + innoculum.

 

Toxicity control attained 81% degradation after 28 -days, which demonstrates the non-inhibitory effect of the test substances on the inoculum used in the test.

 

Control: In the flasks containing aniline alone, pH remained roughly at the same level, which suggests that the final form of nitrogen is partly one of the acid forms NO2 - or NO3- and partlly the alkaline ion NH4 +. The ThOD corresponds to NO3- and was used for the calculation of the biodegradation rates of aniline. Using the ThOD corresponding to NH4+ would lead to higher degradation rates. In both cases, degradation of aniline exceeds 40% after 7 days and 65% after 14 days. The activity of the innoculum is thus verified and the test is considered as valid.

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The test substance underwent 86% biodegradation after 28 days in the test conditions. Biodegradation started on day 2 and reached 80% at the end of the 10-day window. The substance is therefore considered to be readily biodegradable under the conditions of the test.

Executive summary:

The ready biodegradation potential of the test substance, Amyl salicylate, was determined using the OECD guideline 301F (Manometric respirometry test) over a test period of 28-days.

Amyl salicylate attained 86% biodegradation after 28-days under the conditions of the test. Biodegradation started on day 2 and reached 80% at the end of the 10 -day window. Thus, Amyl salicylate is considered to be readily biodegradable.

The substance was exposed to innoculum, prepared from predominantly domestic sewage, at nominal concentration of 100mg/L at approximately 22 degC.

A further similar test sample was used as a toxicity control, which attained greater than 80% degradation after 28-days, thus confirming the non-inhibitory effect of the test substance on the innoculum. A control sample of mineral medium, innoculum and aniline confirmed the actvity of the innoculum used.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions. A reference substance was not included in the study but the high level of degradation seen for the test item confirms that the inoculum was viable)

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

yes

Remarks:

Modified by using sealed test vessels to allow assessment of volatile substances.

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

- Name of test material (as cited in study report): Amyl salicylate
- Molecular weight (if other than submission substance): 208.3
- Structural formula attached as image file (if other than submission substance): see Fig.
- Physical state: liquid
- Analytical purity: 100% nominal
- Lot/batch No.: R456
- Storage condition of test material: Store at room temperature in the dark.
- Other: Percentage Carbon = 69.1%

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, industrial, non-adapted

Duration of test (contact time):

28 d

Initial conc.:

10 mg/L

Based on:

other: Organic carbon

Parameter followed for biodegradation estimation:

CO2 evolution

Parameter:

% degradation (CO2 evolution)

Value:

27.2

Sampling time:

4 d

Parameter:

% degradation (CO2 evolution)

Value:

66.9

Sampling time:

14 d

Parameter:

% degradation (CO2 evolution)

Value:

81.3

Sampling time:

28 d

Conclusions:

The substance is considered to be readily biodegradable after attaining 81.3% degradation within 28-days in a CO2 evolution ready biodegradation study and passing the 10 day window criterion.

Executive summary:

The substance is considered to be readily biodegradable after attaining 81.3% degradation within 28-days in a CO2 evolution ready biodegradation study and passing the 10 -day window criterion.

Description of key information

Readily biodegradable. The test substance exceeded the pass level of 60% within 28 days in two ready biodegradability tests. The  10-day window criterion was met in both studies. 

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Type of water:

freshwater

Additional information

The ready biodegradation potential of the test substance, Amyl salicylate, was determined using the OECD guideline 301F over a test period of 28-days (key study). Amyl salicylate attained 86% biodegradation after 28-days under the conditions of the test. Biodegradation started on day 2 and reached 80% at the end of the 10-day window. Thus, Amyl salicylate is considered to be readily biodegradable.

The substance was exposed to innoculum, prepared from predominantly domestic sewage, at nominal concentration of 100mg/L at approximately 22 degC. A further similar test sample was used as a toxicity control, which attained greater than 80% degradation after 28-days, thus confirming the non-inhibitory effect of the test substance on the innoculum.

In a supporting CO2 evolution ready biodegradation study, the substance attained 81.3% degradation within 28-days in a CO2 evolution ready biodegradation study. The 10 day window criterion was also met.