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EC number: 700-960-7 | CAS number: 68512-30-1
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 Aug. - 08 Oct. 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Samples for TOC-analysis were taken at 0 h and 72 h: TOC-Analyser (DIMA-TOC 100).
Measurements of the cell concentrations per mL: at time 24 h, 48 h and 72 h by optical densitometry at 525 nm - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Water Accommodated Fractions (WAFs) prepared by stirring various amounts of the test item in Alga-medium
for 48 h with magnetic stirrers (open conditions). The WAFs were prepared in 2-L glass beakers.
The beakers were equipped with a glass tube that allowed the separation of the water phase by siphoning.
The test loading rate of Novares LA 100 was weighed in a weighing scoop, that afterwards was placed into the glass beaker.
The beakers were then filled with Alga-medium.
Mixing was carried out at a speed that was slow enough not to cause dispersion or emulsification of the undissolved fraction
of the test item. To ensure this, the vortex developed at the surface by stirring was set at ~ 10 % of the water depth.
After stirring for 48 h the WAFs were allowed to stand for 1 h before use to facilitate phase separation.
The extracts gained with this method were clear.
- Pre-conditioning: The test vessels of the Definite Alga-Test were rinsed with control media and WAFs for ca. 0.5 h before the definite
start of the tests to facilitate adsorption of the test item to the glass walls of the beakers.
Control media and WAFs were then discarded, and the test vessels were refilled again with control media and WAFs, respectively.
- Controls: without test material (blank; Alga medium) - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: unicellular green alga
- Strain: SAG 86.81
- Source: Albrecht von Haller Institut für Pflanzenwissenschaften der Universität Göttingen
- Age of inoculum (at test initiation): ca. 72 h
[Pre-culture: Incubation in 250 mL Erlenmeyer flasks at ± 25 °C on a shaker
(80 - 120 rpm), ca. 72 h, ca. 10000 lux (Gro-Lux fluorescent tubes), permanent illumination]
- Method of cultivation: Sterile on agar tubes (OECD-Medium, 0.8 % Agar), transferred to a new agar tube every 2 months - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- none
- Hardness:
- no data specified: Compositions of stock solutions given.
- Test temperature:
- 23.1 - 24.4 °C (25 +-2°C)
- pH:
- initial (with algae): 8.0 - 8.1
final (with algae): 10.2 (control); 9.4 (5 mg/L); 9.0 (10 mg/L); 8.7 (25 mg/L); 8.5 (50 mg/L); 8.4 (100 mg/L) - Salinity:
- no data specified: Compositions of stock solutions given.
- Nominal and measured concentrations:
- Pre-test:
Time [h] Control 100 mg/L loading
==========================================
24 (stirring) 0.1 3.1 mg C/mL (TOC of WAFs)
48 (stirring) 1.0 4.5 mg C/mL (TOC of WAFs)
==========================================
Main test series:
Time [h] Control 5 mg/L 10 mg/L 25 mg/L 50 mg/L 100 mg/L (loading)
==================================================================
0 7.6 1.8 n.d. 3.4 n.d. 4.7 mg C/L (TOC of WAF)
72 1.2 1.6 n.d 2.7 n.d. 4.4 mg C/L (TOC of WAF)
===================================================================
Note: Control value of time 0 is considered to be an artefact. TOC values include background values. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL-Erlenmeyer
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 100 mL
- Aeration: no
- Initial cells density: approx. 5000 cells/mL
- Control end cells density: approx. 278800 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted from deionised water and mineral stock solutions
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: permanent
- Light intensity and quality: approx. 10000 Lux (Gro-Lux fluorescent tubes)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: densitometrically at 525 nm (turbidity)
- Chlorophyll measurement: no
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.0 - 2.5
- Range finding study
- Results used to determine the conditions for the definitive study: based on results from daphnia test - Key result
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Remarks:
- effective loading
- Effect conc.:
- ca. 6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Novares LA 100
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Remarks:
- effective loading
- Effect conc.:
- 15 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Novares LA 100
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Remarks:
- effective loading
- Effect conc.:
- >= 3 - <= 4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Novares LA 100
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Remarks:
- effective loading
- Effect conc.:
- 8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Novares LA 100
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- < 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Novares LA 100
- Basis for effect:
- other: biomass and growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOELR
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Novares LA 100
- Basis for effect:
- other: biomass and growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Any stimulation of growth found in any treatment: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Observation of abnormalities (for algal test):
Observations by microscopy at the end of the testing period of 72 h:
Control: Appearance of alga cells was normal, cell proliferations could be observed regularly.
5 mg/L: Comparable to control.
10 mg/L: More medium-sized and large alga cells compared to the control, cell proliferations could be observed regularly
but lower number of cell proliferations.
25 mg/L: Appearance of alga cells comparable to 10 mg/L but cell proliferations could not be observed.
50 mg/L: Very low number of alga cells, cells partly deformed, cell proliferations could not be observed.
100 mg/L: Comparable to 50 mg/L. - Reported statistics and error estimates:
- standard deviations, coefficient of variance
- Validity criteria fulfilled:
- yes
- Remarks:
- 1) Biomass in controls increased 56x in 72 h (nom. >= 16x). 2) Mean var-coeff. for section-spec. growth rates: 33.9 % (nom. ≤ 35%). 3) Var-coeff. of av. spec. growth rates for the whole period in replicate controls: 2.6% (nom. value ≤ 7%).
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13 Sep. - 09 Oct. 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Novares LA 700, (phenol, methylstyrenated)
- Lot/batch No.: 23861
- Composition of test material: composition is specified in IUCLID Sect. 13 - Assessment reports under Certificate of Analysis_Novares LA 700_phenol, methylstyrenated
- Stability under test conditions: no measured data; based on chemical structure assumed to be stable
- Storage condition of test material: room temperature, exclusion of light - Analytical monitoring:
- yes
- Details on sampling:
- Samples for TOC-analysis were taken at 0 h and 72 h: TOC-Analyser (DIMA-TOC 100).
Measurements of the cell concentrations per mL: at time 24 h, 48 h and 72 h by optical densitometry at 525 nm - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Water Accommodated Fractions (WAFs) prepared by stirring various amounts of the test item in Alga-medium
for 24 h with magnetic stirrers (open conditions). The WAFs were prepared in 2-L glass beakers.
The beakers were equipped with a glass tube that allowed the separation of the water phase by siphoning.
The test loading rate of Novares LA 700 was weighed in a weighing scoop, that afterwards was placed into the glass beaker.
The beakers were then filled with Alga-medium.
Mixing was carried out at a speed that was slow enough not to cause dispersion or emulsification of the undissolved fraction
of the test item. To ensure this, the vortex developed at the surface by stirring was set at ~ 10 % of the water depth.
After stirring for 24 h the WAFs were allowed to stand for 1 h before use to facilitate phase separation.
The extracts gained with this method were clear.
- Pre-conditioning: The test vessels of the Definite Alga-Test were rinsed with control media and WAFs for ca. 0.5 h before the definite
start of the tests to facilitate adsorption of the test item to the glass walls of the beakers.
Control media and WAFs were then discarded, and the test vessels were refilled again with control media and WAFs, respectively.
- Controls: without test material (blank; Alga medium) - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: unicellular green alga
- Strain: SAG 86.81
- Source: Albrecht von Haller Institut für Pflanzenwissenschaften der Universität Göttingen
- Age of inoculum (at test initiation): ca. 72 h
[Pre-culture: Incubation in 250 mL Erlenmeyer flasks at ± 25 °C on a shaker
(80 - 120 rpm), ca. 72 h, ca. 10000 lux (Gro-Lux fluorescent tubes), permanent illumination]
- Method of cultivation: Sterile on agar tubes (OECD-Medium, 0.8 % Agar), transferred to a new agar tube every 2 months - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- none
- Hardness:
- no data specified: Compositions of stock solutions given.
- Test temperature:
- 23.8 - 24.3 °C (25 +-2°C)
- pH:
- initial (with algae): 7.8 - 8.2
final (with algae): 10.1 (control); 10.3 (5 mg/L); 10.3 (10 mg/L); 10.4 (25 mg/L); 10.1 (50 mg/L); 10.3 (100 mg/L); 9.7 (250 mg/L) - Salinity:
- no data specified: Compositions of stock solutions given.
- Nominal and measured concentrations:
- Time [h] Control 5 mg/L 10 mg/L 25 mg/L 50 mg/L 100 mg/L 250 mg/L (loading)
============================================================================
0 1.7 2.2 n.d. n.d. 2.8 n.d. 3.6 mg C/L (TOC of WAF)
72 1.8 2.2 n.d n.d. 2.7 n.d. 3.5 mg C/L (TOC of WAF)
===================================================================
Note: TOC values include background values. / n.d. = not determined - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL-Erlenmeyer
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 100 mL
- Aeration: no
- Initial cells density: approx. 5000 cells/mL
- Control end cells density: approx. 740,000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted from deionised water and mineral stock solutions
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: permanent
- Light intensity and quality: approx. 10000 Lux (Gro-Lux fluorescent tubes)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: densitometrically at 525 nm (turbidity)
- Chlorophyll measurement: no
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.0 - 2.5
- Range finding study
- Results used to determine the conditions for the definitive study: based on results from daphnia test - Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 250 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Novares LA 700
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Novares LA 700
- Basis for effect:
- other: growth rate and biomass
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 178 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Novares LA 700
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- LOELR
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Novares LA 700
- Basis for effect:
- other: biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Any stimulation of growth found in any treatment: yes, at 5 mg/L
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Observation of abnormalities (for algal test):
Observations by microscopy at the end of the testing period of 72 h:
Control: Appearance of alga cells was normal, cell proliferations could be observed regularly.
5 mg/L: Comparable to control.
10 mg/L: Comparable to control.
25 mg/L: Comparable to control.
50 mg/L: More medium-sized and large alga cells compared to the control.
100 mg/L: Comparable to 50 mg/L.
250 mg/L: Appearance of alga cells comparable to 50 mg/L, but lower number of cells. - Reported statistics and error estimates:
- standard deviations, coefficient of variance
- Validity criteria fulfilled:
- yes
- Remarks:
- 1) Biomass in controls increased 149x in 72 h (nom. >= 16x). 2) Mean var-coeff. for section-spec. growth rates: 23.9 % (nom. ≤ 35%). 3) Var-coeff. of av. spec. growth rates for the whole period in replicate controls: 1.5 % (nom. value ≤ 7%).
Referenceopen allclose all
Growth rates are listed below, from 0 -24, 24 - 48, and 48 - 72 h (Table 1) and average from 0 - 72 h (Table 2):
Table 1: Loading rates of the Test item, cells/mL in the test flasks at 24, 48 and 72 h with mean value,
section-by-section average specific growth rate [μ] during observation times 0-24 h, 24-48 h
and 48-72 h, mean value of the average specific growth rate [μ], standard deviation and
coefficient of variation Vc [%], inhibition [%] and mean value of inhibition of the average
specific growth rate [%] during observation times 0-24 h, 24-48 h and 48-72 h (Rep. Tab. 4)
[Note: Negative inhibition means increased growth, apparently stimulation]
Loading rate [mg/L] |
Cells / mL |
Cells / mL Mean value |
Average Specific growth rate [µ] |
Average Specific growth rate [µ] Mean value |
Standard deviation |
Coefficient of variation Vc [%] |
Inhibition of Average Specific [%] |
Inhibition of Average Specific growth rate Mean value [%] |
24 h |
||||||||
Control 1 |
18746 |
19836 |
1.32 |
1.38
|
0.09
|
6.75
|
||
Control 2 |
22015 |
1.48 |
||||||
Control 3 |
18746 |
1.32 |
||||||
5 |
6083 |
7118 |
0.20 |
0.33
|
0.24
|
71.35
|
85.74 |
75.74
|
5 |
6083 |
0.20 |
85.74 |
|||||
5 |
9187 |
0.61 |
55.76 |
|||||
10 |
6083 |
7118 |
0.20 |
0.33
|
0.24
|
71.35
|
85.74 |
75.74
|
10 |
9187 |
0.61 |
55.76 |
|||||
10 |
6083 |
0.20 |
85.74 |
|||||
25 |
6083 |
4042 |
0.20 |
-0.27
|
0.40
|
149.40
|
85.74 |
119.67
|
25 |
3021 |
-0.50 |
136.64 |
|||||
25 |
3021 |
-0.50 |
136.64 |
|||||
50 |
3021 |
4042 |
-0.50 |
-0.27
|
0.40
|
149.40
|
136.64 |
119.67
|
50 |
6083 |
0.20 |
85.74 |
|||||
50 |
3021 |
-0.50 |
136.64 |
|||||
100 |
3021 |
3021 |
-0.50 |
-0.50 |
0 |
0 |
136.64 |
136.64 |
100 |
3021 |
-0.50 |
136.64 |
|||||
100 |
3021 |
-0.50 |
136.64 |
|||||
Loading rate [mg/L] |
Cells / mL |
Cells / mL Mean value |
Average Specific growth rate [µ] |
Average Specific growth rate [µ] Mean value |
Standard deviation |
Coefficient of variation Vc [%] |
Inhibition of Average Specific [%] |
Inhibition of Average Specific growth rate Mean value [%] |
48 h |
||||||||
Control 1 |
46053 |
47253 |
0.90 |
0.87 |
0.12
|
13.85
|
|
|
Control 2 |
46053 |
0.74 |
||||||
Control 3 |
49653 |
0.97 |
||||||
5 |
46053 |
43681 |
2.02 |
1.83
|
0.26
|
14.42
|
-132.58 |
-110.64
|
5 |
42495 |
1.94 |
-123.34 |
|||||
5 |
42495 |
1.53 |
-75.98 |
|||||
10 |
42495 |
44867 |
1.94 |
1.86
|
0.22
|
11.75
|
-123.34 |
-113.71
|
10 |
46053 |
1.61 |
-85.22 |
|||||
10 |
46053 |
2.02 |
-132.58 |
|||||
25 |
32069 |
34359 |
1.66 |
2.20
|
0.46
|
21.07
|
-91.00 |
-152.41
|
25 |
35503 |
2.46 |
-183.11 |
|||||
25 |
35503 |
2.46 |
-183.11 |
|||||
50 |
15518 |
16594 |
1.64 |
1.47
|
0.47
|
31.94
|
-88.02 |
-68.45
|
50 |
15518 |
0.94 |
-7.60 |
|||||
50 |
18746 |
1.83 |
-109.73 |
|||||
100 |
18746 |
13422 |
1.83 |
1.45 |
0.36 |
24.75 |
-109.73 |
-66.38 |
100 |
9187 |
1.11 |
-27.79 |
|||||
100 |
12332 |
1.41 |
-61.62 |
Loading rate [mg/L] |
Cells / mL |
Cells / mL Mean value |
Average Specific growth rate [µ] |
Average Specific growth rate [µ] Mean value |
Standard deviation |
Coefficient of variation Vc [%] |
Inhibition of Average Specific [%] |
Inhibition of Average Specific growth rate Mean value [%] |
72 h |
||||||||
Control 1 |
305342 |
278840 |
1.89 |
1.77
|
0.14
|
8.14
|
|
|
Control 2 |
282272 |
1.81 |
||||||
Control 3 |
248906 |
1.61 |
||||||
5 |
206731 |
229445 |
1.50 |
1.66
|
0.13
|
8.14
|
15.27 |
6.51
|
5 |
243490 |
1.75 |
1.50 |
|||||
5 |
238115 |
1.72 |
2.76 |
|||||
10 |
134777 |
98229 |
1.15 |
0.75
|
0.35
|
46.84
|
34.87 |
57.58
|
10 |
76004 |
0.50 |
71.73 |
|||||
10 |
83905 |
0.60 |
66.15 |
|||||
25 |
9187 |
8153 |
-1.25 |
-1.46
|
0.27
|
18.70
|
170.54 |
182.12
|
25 |
9187 |
-1.35 |
176.28 |
|||||
25 |
6083 |
-1.76 |
199.54 |
|||||
50 |
6083 |
4042 |
-0.94 |
-1.47
|
0.47
|
31.94
|
152.84 |
182.72
|
50 |
3021 |
-1.64 |
192.34 |
|||||
50 |
3021 |
-1.83 |
203.00 |
|||||
100 |
3021 |
3021 |
-1.83 |
-1.45 |
0.36 |
24.75 |
203.00 |
181.71 |
100 |
3021 |
-1.11 |
162.76 |
|||||
100 |
3021 |
-1.41 |
179.37 |
Table 2: Loading rate of the Test item, cells/mL in the test flasks at 72 h, average specific growth
rate [μ] over the whole testing period 0-72 h, mean value of the average specific growth
rate [μ], standard deviation and coefficient of variation Vc [%], inhibition [%] and mean value
of inhibition [%] of the average specific growth rate [μ] (Rep. Tab. 5)
Loading rate [mg/L] |
Cells / mL |
Average Specific growth rate [µ] |
Average Specific growth rate [µ] Mean value |
Standard deviation |
Coefficient of variation Vc [%] |
Inhibition of Average Specific growth rate [%] |
Inhibition of Average Specific growth rate Mean value [%] |
72 h |
|||||||
Control 1 |
305342 |
1.37 |
1.34 |
0.03 |
2.57 |
||
Control 2 |
282272 |
1.34 |
|||||
Control 3 |
248906 |
1.30 |
|||||
5 |
206731 |
1.24 |
1.27
|
0.03
|
2.32
|
7.36 |
4.83
|
5 |
243490 |
1.30 |
3.29 |
||||
5 |
238115 |
1.29 |
3.84 |
||||
10 |
134777 |
1.10 |
0.98
|
0.10
|
10.40
|
18.01 |
26.69
|
10 |
76004 |
0.91 |
32.27 |
||||
10 |
83905 |
0.94 |
29.80 |
||||
25 |
9187 |
0.20 |
0.16
|
0.08
|
50.54
|
84.86 |
88.28
|
25 |
9187 |
0.20 |
84.86 |
||||
25 |
6083 |
0.07 |
95.12 |
||||
50 |
6083 |
0.07 |
-0.09
|
0.13
|
149.40
|
95.12 |
106.73
|
50 |
3021 |
-0.17 |
112.54 |
||||
50 |
3021 |
-0.17 |
112.54 |
||||
100 |
3021 |
-0.17 |
-0.17 |
0 |
0 |
112.54 |
112.4 |
100 |
3021 |
-0.17 |
112.54 |
||||
100 |
3021 |
-0.17 |
112.54 |
Growth rates are listed below, from 0 - 24, 24 - 48, and 48 - 72 h (Table 1) and average from 0 - 72 h (Table 2):
Table 1: Loading rates of the Test item, cells/mL in the test flasks at 24, 48 and 72 h with mean value,
section-by-section average specific growth rate [μ] during observation times 0-24 h, 24-48 h
and 48-72 h, mean value of the average specific growth rate [μ], standard deviation and
coefficient of variation Vc [%], inhibition [%] and mean value of inhibition of the average
specific growth rate [%] during observation times 0-24 h, 24-48 h and 48-72 h (Rep. Tab. 4)
[Note: Negative inhibition means increased growth, apparently stimulation]
Loading rate [mg/L] |
Cells / mL |
Cells / mL Mean value |
Average Specific growth rate [µ] |
Average Specific growth rate [µ] Mean value |
Standard deviation |
Coefficient of variation Vc [%] |
Inhibition of Average Specific [%] |
Inhibition of Average Specific growth rate Mean value [%] |
24 h |
||||||||
Control 1 |
25325 |
32124 |
1.62 |
1.84
|
0.22
|
11.71
|
||
Control 2 |
38979 |
2.05 |
||||||
Control 3 |
32069 |
1.86 |
||||||
5 |
38979 |
37820 |
2.05 |
2.02
|
0.05
|
2.67
|
-11.32 |
-9.63
|
5 |
35503 |
1.96 |
-6.25 |
|||||
5 |
38979 |
2.05 |
-11.32 |
|||||
10 |
35503 |
32083 |
1.96 |
1.86
|
0.11
|
5.76
|
-6.25 |
-0.56
|
10 |
28677 |
1.75 |
5.32 |
|||||
10 |
32069 |
1.86 |
-0.74 |
|||||
25 |
32069 |
34359 |
1.86 |
1.93
|
0.06
|
3.05
|
-0.74 |
-4.42
|
25 |
35503 |
1.96 |
-6.25 |
|||||
25 |
35503 |
1.96 |
-6.25 |
|||||
50 |
32069 |
32083 |
1.86 |
1.86
|
0.11
|
5.76
|
-0.74 |
-0.56
|
50 |
35503 |
1.96 |
-6.25 |
|||||
50 |
28677 |
1.75 |
5.32 |
|||||
100 |
28677 |
28690 |
1.75 |
1.74
|
0.12
|
6.78
|
5.32 |
5.55
|
100 |
32069 |
1.86 |
-0.74 |
|||||
100 |
25325 |
1.62 |
12.06 |
|||||
250 |
25325 |
24222 |
1.62 |
1.58 |
0.08 |
5.13 |
12.06 |
14.59 |
250 |
25325 |
1.62 |
12.06 |
|||||
250 |
22015 |
1.48 |
19.65 |
|||||
Loading rate [mg/L] |
Cells / mL |
Cells / mL Mean value |
Average Specific growth rate [µ] |
Average Specific growth rate [µ] Mean value |
Standard deviation |
Coefficient of variation Vc [%] |
Inhibition of Average Specific [%] |
Inhibition of Average Specific growth rate Mean value [%] |
48 h |
||||||||
Control 1 |
186635 |
219590 |
2.00 |
1.93 |
0.07
|
3.49
|
|
|
Control 2 |
265403 |
1.92 |
||||||
Control 3 |
206731 |
1.86 |
||||||
5 |
222237 |
227915 |
1.74 |
1.79
|
0.09
|
5.10
|
9.64 |
7.00
|
5 |
201645 |
1.74 |
9.84 |
|||||
5 |
259863 |
1.90 |
1.52 |
|||||
10 |
130311 |
147198 |
1.30 |
1.52
|
0.20
|
12.94
|
32.50 |
21.09
|
10 |
139285 |
1.58 |
17.96 |
|||||
10 |
171997 |
1.68 |
12.81 |
|||||
25 |
153057 |
148852 |
1.56 |
1.46
|
0.20
|
13.49
|
18.87 |
24.36
|
25 |
121502 |
1.23 |
36.13 |
|||||
25 |
171997 |
1.58 |
18.09 |
|||||
50 |
134777 |
141036 |
1.44 |
1.48
|
0.04
|
2.87
|
25.47 |
23.25
|
50 |
162444 |
1.52 |
21.06 |
|||||
50 |
125886 |
1.48 |
23.21 |
|||||
100 |
91970 |
108777 |
1.17 |
1.33
|
0.16
|
12.36
|
39.50 |
30.93
|
100 |
121502 |
1.33 |
30.85 |
|||||
100 |
112858 |
1.49 |
22.43 |
|||||
250 |
79934 |
77328 |
1.15 |
1.16 |
0.12 |
10.50 |
40.33 |
39.69 |
250 |
72116 |
1.05 |
45.68 |
|||||
250 |
79934 |
1.29 |
33.06 |
|||||
Loading rate [mg/L] |
Cells / mL |
Cells / mL Mean value |
Average Specific growth rate [µ] |
Average Specific growth rate [µ] Mean value |
Standard deviation |
Coefficient of variation Vc [%] |
Inhibition of Average Specific [%] |
Inhibition of Average Specific growth rate Mean value [%] |
72 h |
||||||||
Control 1 |
689559 |
745202 |
1.31 |
1.23
|
0.17
|
13.90
|
|
|
Control 2 |
747421 |
1.04 |
||||||
Control 3 |
798628 |
1.35 |
||||||
5 |
697701 |
906541 |
1.14 |
1.36
|
0.36
|
26.27
|
7.08 |
-10.55
|
5 |
1188331 |
1.77 |
-44.06 |
|||||
5 |
833591 |
1.17 |
5.33 |
|||||
10 |
513688 |
658879 |
1.37 |
1.45
|
0.24
|
16.82
|
-11.41 |
-18.17
|
10 |
492359 |
1.26 |
-2.55 |
|||||
10 |
970591 |
1.73 |
-40.54 |
|||||
25 |
513688 |
740153 |
1.21 |
1.58
|
0.43
|
26.93
|
1.66 |
-28.62
|
25 |
942447 |
2.05 |
-66.38 |
|||||
25 |
764324 |
1.49 |
-21.14 |
|||||
50 |
789990 |
587766 |
1.77 |
1.40
|
0.32
|
22.69
|
-43.63 |
-13.83
|
50 |
542705 |
1.21 |
2.03 |
|||||
50 |
430603 |
1.23 |
0.12 |
|||||
100 |
550063 |
508325 |
1.79 |
1.53
|
0.30
|
19.93
|
-45.26 |
-24.08
|
100 |
602721 |
1.60 |
-30.07 |
|||||
100 |
372191 |
1.19 |
3.08 |
|||||
250 |
282272 |
288404 |
1.26 |
1.31 |
0.07 |
5.54 |
-2.47 |
-6.67 |
250 |
259863 |
1.28 |
-4.11 |
|||||
250 |
323078 |
1.40 |
-13.44 |
Table 2: Loading rate of the Test item, cells/mL in the test flasks at 72 h, average specific growth
rate [μ] over the whole testing period 0-72 h, mean value of the average specific growth
rate [μ], standard deviation and coefficient of variation Vc [%], inhibition [%] and mean value
of inhibition [%] of the average specific growth rate [μ] (Rep. Tab. 5)
Loading rate [mg/L] |
Cells / mL |
Average Specific growth rate [µ] |
Average Specific growth rate [µ] Mean value |
Standard deviation |
Coefficient of variation Vc [%] |
Inhibition of Average Specific growth rate [%] |
Inhibition of Average Specific growth rate Mean value [%] |
72 h |
|||||||
Control 1 |
689559 |
1.64 |
1.67 |
0.02 |
1.47 |
||
Control 2 |
747421 |
1.67 |
|||||
Control 3 |
798628 |
1.69 |
|||||
5 |
697701 |
1.65 |
1.73
|
0.09
|
5.24
|
1.28 |
-3.45
|
5 |
1188331 |
1.82 |
-9.36 |
||||
5 |
833591 |
1.71 |
-2.28 |
||||
10 |
513688 |
1.54 |
1.61
|
0.13
|
7.87
|
7.40 |
3.44
|
10 |
492359 |
1.53 |
8.25 |
||||
10 |
970591 |
1.76 |
-5.32 |
||||
25 |
513688 |
1.54 |
1.66
|
0.10
|
6.21
|
7.40 |
0.71
|
25 |
942447 |
1.75 |
-4.73 |
||||
25 |
764324 |
1.68 |
-0.54 |
||||
50 |
789990 |
1.69 |
1.58
|
0.10
|
6.47
|
-1.20 |
5.34
|
50 |
542705 |
1.56 |
6.30 |
||||
50 |
430603 |
1.49 |
10.93 |
||||
100 |
550063 |
1.57 |
1.53
|
0.09
|
5.56
|
6.03 |
8.03
|
100 |
602721 |
1.60 |
4.21 |
||||
100 |
372191 |
1.44 |
13.84 |
||||
250 |
282272 |
1.34 |
1.35 |
0.04 |
2.71 |
19.37 |
19.02 |
250 |
259863 |
1.32 |
21.02 |
||||
250 |
323078 |
1.39 |
16.67 |
Description of key information
Due to substance properties, toxicity to aquatic algae and cyanobacteria is determined based on loadings. The lowest EL50 value of two tests using two technical products of 'Oligomerisation and alkylation reaction products of 2-phenylpropene and phenol' is selected to characterise the acute toxicity to algae and cyanobacteria.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 15 mg/L
- EC10 or NOEC for freshwater algae:
- 6 mg/L
Additional information
The substance 'Oligomerisation and alkylation reaction products of 2-phenylpropene and phenol' (OAPP) (previous name phenol, methylstyrenated) is a UVCB substance with a complex and within limits variable composition. Water solubility is low and not clearly defined due to the differing solubility of individual components. Depending on the quantity used, concentration of material dissolved in saturated water samples is variable. Therefore, water accommodated fractions (WAFs) have been used in aquatic toxicity tests. Thus, results are presented as loadings (EL50, EL10).
Toxicity to aquatic algae and cyanobacteria has been tested in valid GLP guideline studies (OECD TG 201) with two different technical products of OAPP (LA 100 and LA 700). The two products are composed of the same constituents and differ only in the quantitative composition of components. They are subsumed under the same EC Number (700-960-7).
The solubility of the two technical products is somewhat different. Therefore, it can be expected that aqueous effect levels differ to some degree. For reasons of comprehensive protection, the lowest EL50 / EL10 values (15 mg/L / ca. 6 mg/L, based on growth rate) (LA 100) are selected as key values for the chemical safety assessment.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
