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Short-term toxicity to aquatic invertebrates

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In a key static 48 -hour short-term Daphnia magna limit test (OECD 202; KS = 2), 10 animals/loading were exposed to the WAF of a highly refined base oil (HT350) at a nominal concentrations of 100 mg/L. The 48 hour LL50 was >100 mg/L and the NOEL was ≥100 mg/L (Petro-Canada, 2008f). 

In four supporting 48-hour short-term Daphnia magna limit tests, 10 animals/loading were exposed to highly refined base oils (HT70, HT100, HT200, and N60LHT) at nominal concentrations of 100 mg/L. The 48 hour LL50 was >100 mg/L and the NOEL was ≥ 100 mg/L (Petro-Canada, 2008g, h, i, j). 

Supporting data estimated from the PETROTOX computer model show no acute toxicity of highly refined base oil to aquatic invertebrates at or below its maximum attainable water solubility (Redman et al., 2010b). These predictions support the applied read across.