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EC number: 931-257-5 | CAS number: -
The aim of the study was to investigate acute toxic effects of the test substance, Fluidized Bed Combustion (FBC) Bottom Ash, after a single oral administration to Wistar rats.
The testing was performed according to the Method B.1 tris: Acute Oral Toxicity - Acute Toxic Class Method, Council Regulation (EC) No.440/2008, published in O.J. L 142, 2008.
The test substance was administered in a single dose as solution in vehicle (olive oil), given orally via gavage to two groups of three female Wistar rats.
The dosing was performed sequentially in two groups of three females: group No. 1 - first step using the starting dose of 2000 mg/kg of body weight and group No.2 - second step using the same dose.
The test substance administered at the dose of 2000 mg/kg caused no death of animals. Clinical signs of intoxication (piloerection) were observed in all six animals at the 3 hours after application only.
No pathologic macroscopic changes were diagnosed during pathological examination in all animals
According to the study results the value of LD50 of the test substance for female rats is higher than 2000 mg/kg of body weight.
The classification of the test substance toxicity was performed according to the Directive 67/548/EEC, Annex VI. part 3.1.5. and 3.2.
Based on the test results and according to the EC criteria for classification and labelling requirements for dangerous substances and preparations the test substance Fluidized Bed Combustion (FBC) Bottom Ash did not fall into any of quoted categories of toxicity and has no obligatory labelling requirement in this respect.
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