Sodium 2-methyl-2-[(1-oxoallyl)amino]propanesulphonate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

November 1-3, 1983

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented report of a guideline study conducted according to GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EPA-660/3-75-009, "Methods for acute toxicity with fish, macrovertebrates, and amphibians"

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Sodium 2-methyl-2-[(1-oxoallyl)amino]propanesulphonate

EC Number:

225-948-4

EC Name:

Sodium 2-methyl-2-[(1-oxoallyl)amino]propanesulphonate

Cas Number:

5165-97-9

Molecular formula:

C7H13NO4S.Na

IUPAC Name:

sodium 2-(acryloylamino)-2-methylpropane-1-sulfonate

Details on test material:

- Name of test material (as cited in study report): OS #48933F
- Physical state: liquid
- Analytical purity: no data
- Impurities (identity and concentrations): no data
- Composition of test material, percentage of components: 100%
- Purity test date: no data
- Lot/batch No.: no data
- Expiration date of the lot/batch: no data
- Stability under test conditions: stable
- Storage condition of test material: no data

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Dilution
- Chemical name of vehicle: water

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnid
- Strain: Daphnia magna
- Source: in house
- Age at study initiation: ≤24 hours
- Weight at study initiation: no data
- Feeding during test: no
ACCLIMATION
- Acclimation period: yes
- Acclimation conditions: same as test
- Type and amount of food: green algae (Ankistrodemus sp.) and yeast
- Feeding frequency: once daily
- Health during acclimation (any mortality observed): no data

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

160 - 180 mg/L as CaCO3

Test temperature:

20±1°C

pH:

7.9 - 8.3

Dissolved oxygen:

>60% saturation

Nominal and measured concentrations:

Nominal concentrations: control; 130, 220, 360, 600 and 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: closed
- Material, size, headspace, fill volume: beaker containing each 200 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: well water fortified according to test protocol and filtered through a carbon filter and an Amberlite ZAD-7 resin column to remove any potential organic contaminants
- Alkalinity: 110- 130 mg/L
- Conductivity: 400 - 600 microhos/cm
OTHER TEST CONDITIONS
- Photoperiod: 16 hours of light and 8-hours of darkness

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

There was no effect on Daphnia magna at the highest test concentration of 1000 mg/l.