Registration Dossier
Registration Dossier
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EC number: 915-730-3 | CAS number: -
Endpoint summary
Administrative data
Description of key information
Additional information
Four species representing three different levels in the aquatic food chain were tested. For the short term tests water accommodated fractions (WAF) of14C-labeled OTNE were prepared: the treatment solutions were stirred for 20 hours and left to settle for one hour. The water fractions were used in closed test vessels with minimal headspace. For the longer term tests flow-through systems were usedwith14C-labeled OTNE dissolved in acetone. Blank and solvent controls were included. The actual concentrations were measured using Liquid Scintillation Counting (LSC). The results were expressed based on the measured concentrations. The results for micro-organisms are taken from the biodegradation test.
Test species |
Guideline |
Results (measured, <95% confidence limits>) |
Remarks |
Algae (Scenedesmus subspicatus) |
Comparable to OECD 201 |
72h-NOEC ≥ 2.6 mg/l 72h-EbC50> 2.6 mg/l 72h-ErC50> 2.6 mg/l |
Kranzfelder et al 1998c Measured: 0.53 – 2.6 mg/l; 100-109% of nominal |
Daphnia magna |
Comparable to OECD 202 |
48h-EC50= 1.38 mg/l <1.32 – 1.44> |
Kranzfelder et al 1998a Measured: 0.51 – 2.6 mg/l; 100-106% of nominal |
Daphnia magna |
OECD 211 |
21d-EC10 (repr.) = 0.044 mg/l 21d-EC50(repr.) = 0.285 mg/l <0.122 – 0.663> |
Bogers 2002 Measured: 0.013 – 0.45 mg/l; 48-65% of nominal |
Bluegill (Lepomis macrochirus) |
Comparable to OECD 203 |
96h-LC50= 1.3 mg/l <1.2 – 1.5> |
Kranzfelder et al 1998b Measured: 0.48 – 2.72 mg/l; 95 – 115% of nominal |
Zebra fish (Danio rerio) |
OECD 210 |
30d-NOEChatch ≥ 0.54 mg/l 30d-NOECsurv. during yolk sac period ≥ 0.54 mg/l 30d-NOEC surv. larvae = 0.30 mg/l 30d-NOEC growth = 0.16 mg/l |
Bogers 2001 Prehatch: fresh 61-115% of nominal, 28-63% at renewal Larvae in flow-through: 59-113% Average exposure: 79-66% of nominal |
Micro-organisms (activated sludge) |
OECD 301F |
NOEC > 100 mg/l (respiration) |
Rudio 1995 Toxicity control in test for ready biodegradation |
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