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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vivo

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Administrative data

Endpoint:
in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report date:
2000

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
GLP compliance:
yes (incl. QA statement)
Type of assay:
micronucleus assay

Test material

Constituent 1
Chemical structure
Reference substance name:
6-phenyl-1,3,5-triazine-2,4-diyldiamine
EC Number:
202-095-6
EC Name:
6-phenyl-1,3,5-triazine-2,4-diyldiamine
Cas Number:
91-76-9
Molecular formula:
C9H9N5
IUPAC Name:
6-phenyl-1,3,5-triazine-2,4-diamine

Test animals

Species:
mouse
Strain:
NMRI
Sex:
male/female

Administration / exposure

Route of administration:
oral: gavage
Doses / concentrations
Remarks:
Doses / Concentrations:
male mice; 75, 150, 300 mg/kg b.w. |female mice; 50, 100, 200 mg/kg b.w.
Basis:

Results and discussion

Test results
Key result
Sex:
male/female
Genotoxicity:
negative
Toxicity:
no effects
Positive controls validity:
valid
Additional information on results:
This substance was not mutagenic in bacteria [OECD TG 471]. It induced chromosomal aberration in CHL/IU cells with and without an exogenous metabolic activation system even under the soluble concentrations. It also gave a positive response in the human lymphocytes tested [OECD TG 473] and the mouse lymphoma TK assay [OECD TG 476] but only under the insoluble dose levels. The cytogenetic effect observed in in vitro assays however, could not be reproduced in the micronucleus tests in vivo [OECD TG 474].

Any other information on results incl. tables

STATISTICAL RESULTS:
-The highest dose (300 mg/kg for males and 200 mg/kg b.w. for females) was estimated by five pre-experiments to the suitable since higher concentrations were lethal. After treatment with the test item the number of NCEs was not substantially increased as compared to the mean value of NCEs of the vehicle control thus indicating that this substance at the indicated concentrations had no cytotoxic effectiveness in the bone marrow.
There was no biologically and statistically relevant enhancement in the frequency of the detected micronuclei after administration of the test item at any dose level or sampling time as compared to vehicle controle. 40 mg/kg b.w. cyclophosphamide administered orally was used as positive control which showed a substantial increase of induced micronucleus frequency.

-micronucleus test results
(A)Male animals

Test group  Dose      Sampling     Sampling       NCEs per
--- mg/kg b.w. time (hr) micronuclei(%) 2000 PCEs
vehicle 0 24 0.02 1566
BG 75 24 0.08 1562
BG 150 24 0.09 1814
BG 300 24 0.02 1582
CP 40 24 1.11 2033

Vehicle 0 48 0.01 1694
BG 75 48 0.02 1639
BG 150 48 0.08 1795
BG 300 48 0.07 1755

Vehicle 0 72 0.03 1610
BG 75 72 0.04 1417
BG 150 72 0.03 1549
BG 300 72 0.02 1645

(B)Female animals

Test group Dose Sampling Sampling NCEs per
--- mg/kg b.w. time (hr) micronuclei(%) 2000 PCEs
vehicle 0 24 0.03 1652
BG 50 24 0.03 1563
BG 100 24 0.09 1463
BG 200 24 0.04 1889
CP 40 24 0.98 1755

Vehicle 0 48 0.01 2113
BG 50 48 0.04 1727
BG 100 48 0.01 1628
BG 200 48 0.02 1945

Vehicle 0 72 0.02 1577
BG 50 72 0.02 1408
BG 100 72 0.04 1638
BG 200 72 0.01 1492

BG = benzoguanamine (2,4-diamino-6-phenyl-1,3,5-triazine)
CP = cyclo phosphamide

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): negative
not genotoxic in vivo
Executive summary:

In a CD-1mousebone marrow micronucleus assay, 5 male and femalewere treated oral gavagewith this substanceat doses of 0, 75, 150, 300 mg/kg bw in male mice and 0, 50, 100, 200 mg/kg/bw.  Bone marrow cells were harvested at 24, 48, 72Hrpost-treatment.  The vehicle was corn oil. 

 

There were nosigns of toxicityduring the study.The positive control induced the appropriate response. There was nota significant increase in the frequency of micronucleated polychromatic erythrocytes in bone marrow after any treatment time.

 

This study satisfiesthe requirement for Test Guideline OECD 474 forin vivocytogenetic mutagenicity data.