7-methyl-3-methyleneocta-1,6-diene

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

August 2010

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Study conducted similarly to OECD 203: some concentrations were obtained by extrapolation of the calibration curve

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to other study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Qualifier:

according to guideline

Guideline:

ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 1: Static Method)

Qualifier:

according to guideline

Guideline:

ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 2: Semi-static method)

Qualifier:

according to guideline

Guideline:

other: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, December 14, 2000

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Vapour pressure: 250-270 Pa at 298.15K
- Water solubility (under test conditions): between 0.080 and 1.090 mg/L (OECD 105 in milli-Q purified water at 20°C)
- Stability in water: unknown
- Specific gravity: 0.794 g/mL at 20°C

Analytical monitoring:

yes

Details on sampling:

- Concentrations: from all test concentrations and the control
- Sampling method: samples were taken from the approximate centre of the test vessels
- Frequency: 1. at the start of the test and after 72 hours from the freshly prepared solutions: 2. At the first renewal (t=24h) and the end of the test from the 24-hour old solutions
- Volume: varying from 0.5 to 10 mL
- Sample storage conditions before analysis: stored in a freezer

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Water Accomodated Fraction (WAF)
- Preparation of test solutions started with loading rates of 1.0, 10 and 100 mg/l for the range-finding test and loading rates of 0.46, 1.0, 2.2, 4.6 and 10 mg/l for the final test applying one day of magnetic stirring without illumination. After a stabilisation period of one day without illumination the WAFs were taken out and used for testing.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no vehicle
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no evidence of undissolved material; the final test solutions were all clear and colourless

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Carp
- Source: Zodiac, porefacc, "De Haar Vissen", Wageningen, University and Research Centre, The Netherlands
- Age at study initiation (mean and range, SD): no data
- Length at study initiation (length definition, mean, range and SD): range-finding test: 2.3 ± 0.1 cm / final test: 2.3 ± 0.2 cm
- Weight at study initiation (mean and range, SD): range-finding test: 0.30 ± 0.04 g/ final test: 0.31 ± 0.06 g
- Feeding during test: no feeding from 24 hours prior to the test and during the total test period

ACCLIMATION
- Acclimation period: at least 12 days after delivery
- Acclimation conditions (same as test or not): same as test; adjusted ISO medium formulated using RO-water with the following composition: CaCl2.2H2O 211.5 mg/L; MgSO4.7H20 88.8 mg/L; NaHCO3 46.7 mg/L; KCl 4.2 mg/L
- Type and amount of food: pelleted fish food, no data on amount
- Feeding frequency: daily
- Health during acclimation (any mortality observed): mortality during the seven days prior to the start of the test was less than 5%

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

No post exposure observation period

Hardness:

180 mg/l CaCO3

Test temperature:

at the start of the test: 20.3 °C
During test: 20.0 - 20.5 °C

pH:

7.7 ± 0.3

Dissolved oxygen:

at the start of the test: 9.0 mg/l
During test: 7.5 -9.1 mg/l

Salinity:

not relevant

Nominal and measured concentrations:

Range finding study: WAF prepared at loading rates of 1.0, 10, 100 mg/L
Final test: WAF prepared at loading rates of 0.46, 1.0, 2.2, 4.6 & 10 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: all-glass vessels
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 9.2 litres containing 9L of test solution
- Aeration: not aerated during the test
- Renewal rate of test solution (frequency/flow rate): daily renewal
- No. of organisms per vessel: 7 fishes/ per concenrtation and control
- No. of vessels per concentration (replicates): no data
- No. of vessels per control (replicates): no data
- No. of vessels per vehicle control (replicates): no vehicle control
- Biomass loading rate: 0.24 g fish/l

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap-water purified by reverse osmosis (source: GEON Waterbehandeling, Berkel-Enschot, The Netherlands)
- Culture medium different from test medium: no
- Water quality parameters were kept within the optimum limits for the respective fish species
- Intervals of water quality measurement: pH, nitrate and nitrite concentration and ammonia concentration: once a week. Temperature: every day. pH and temperature were measured before transferring the fish to the test sytem;

OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment
- Photoperiod: 16 hours photoperiod lux
- Light intensity: 9-10 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality and other effects

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study
- Test concentrations: Range finding study: loading rates of 1.0, 10, 100 mg/l
- Results used to determine the conditions for the definitive study: no mortality or clinical effects observed et the 1.0 mg/l WAF.

See Table 1: Concentrations of the test substance in test medium - final test in section “Any other information on materials and methods incl. tables

Reference substance (positive control):

yes

Remarks:

pentachlorophenol (PCP), tested concentrations 0.10, 0.22 and 0.46 mg/L in ISO-medium

Key result

Duration:

96 h

Dose descriptor:

NOEC

Nominal / measured:

estimated

Conc. based on:

dissolved

Remarks:

average concentration obtained in a WAF prepared at a loading rate of 10 mg/l

Basis for effect:

mortality (fish)

Remarks on result:

other: NOEC = limit of solubility

Duration:

96 h

Dose descriptor:

LC50

Nominal / measured:

estimated

Conc. based on:

dissolved

Remarks:

average concentration obtained in a WAF prepared at a loading rate of 10 mg/l

Basis for effect:

mortality (fish)

Remarks on result:

other: 96h-LC50 > solubility limit

Details on results:

See following Table(s) in "Any other information on results" section:
Table 2: Incidence of mortality and total mortality during the final test
Table 3: Incidence of mortality observed in the reference study

Results with reference substance (positive control):

The 96h-LC50 for carp exposed to PCP was estimated to be 0.15 mg/l. The range of the 96h-LC50 for carp is generally between 0.10 and 0.46 mg/l based on historical data of reference tests.

Reported statistics and error estimates:

no reported statistics and error estimates

Sublethal observations / clinical signs:

Table 2: Incidence of mortality and total mortality during the final test

 

Loading rate^1MYRCENE (mg/L)	Initial number of fish	Cumulative mortality					Total mortality
		3h	24h	48h	72h	96h
Control	7	0	0	0	0	0	0
0.46	7	0	0	0	0	0	0
1.0	7	0	0	0	0	0	0
2.2	7	0	0	0	0	0	0
4.6	7	0	0	0	0	0	0
10	7	0	0	0	0	0	0

1 WAF prepared at the given loading rate 

Table 3: Incidence of mortality observed in the reference study

 

Concentration PCP Nominal (mg/l)	Initial number of fish	Cumulative number of dead fish recorded at various time points after start of exposure						Total mortality (%)
		3h	61/2h	24h	48h	72h	96h
0.10	5	0	0	0	0	0	0	0
0.22	5	0	0	5	5	5	5	100
0.46	5	0	5	5	5	5	5	100

 

 

Validity criteria fulfilled:

yes

Remarks:

mortality of control not exceed 10%, constant conditions of test were maintained, the dissolved oxygen concentration was > 60% of the air saturation

Conclusions:

Due to the very low water solubility of MYRCENE in water, dissolved concentration levels that might be toxic for carp could not be reached.
Therefore, the NOEC was at the limit of solubility.

Executive summary:

In a 96-h acute toxicity study, carp (Cyprinus carpio) were exposed to myrcene at WAFs prepared at loading rates of 0 (control), 0.46, 1.0, 2.2, 4.6 and 10 mg /L under semi-static conditions. Initial concentrations measured during the final test varied but were more in the lower range or below the given water solubility, which may be in accordance with the solubility limit of myrcene in the test medium.

The 96-h LC₅₀ was > limit of solubility. The NOEC value, based on mortality was at the limit of solubility. No sublethal effects were observed.

 

Description of key information

A fish study was conducted with the substance Myrcene according to the OECD guideline 203.

The study was conducted in semi-static conditions. Carp (Cyprinus carpio) were exposed to myrcene at WAFs prepared at loading rates of 0 (control), 0.46, 1.0, 2.2, 4.6 and 10mg /L under semi-static conditions.
The 96-h LC50 was > limit of solubility.
The NOEC value, based on mortality was at limit of solubility. No sublethal effects were observed.