Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 246-836-1 | CAS number: 25321-14-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study without analytical dose verification, study was peer reviewed in OECD SIDS (2004)
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
- Reference Type:
- publication
- Title:
- QSAR study of the toxicity of nitrobenzene derivatives towards Daphnia magna, Chlorella pyrenoidosa and Photobacterium phosphoreum.
- Author:
- Deneer JW, van Leeuwen CJ, Seinen W, Maas-Diepeveen JL, Hermens JLM
- Year:
- 1 989
- Bibliographic source:
- Aquatic Toxicol. 15, 83-98. As cited in: OECD SIDS, Dinitrotoluene (isomers mixture), CAS No. 25321-14-6, 2004
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- 1984
- Deviations:
- yes
- Remarks:
- modifications according to van Leeuwen et al. (1985)
- GLP compliance:
- no
Test material
- Reference substance name:
- 2,3-dinitrotoluene
- EC Number:
- 210-013-5
- EC Name:
- 2,3-dinitrotoluene
- Cas Number:
- 602-01-7
- Molecular formula:
- C7H6N2O4
- IUPAC Name:
- 1-methyl-2,3-dinitrobenzene
- Reference substance name:
- 2,4-dinitrotoluene
- EC Number:
- 204-450-0
- EC Name:
- 2,4-dinitrotoluene
- Cas Number:
- 121-14-2
- Molecular formula:
- C7H6N2O4
- IUPAC Name:
- 1-methyl-2,4-dinitrobenzene
- Reference substance name:
- 2,6-dinitrotoluene
- EC Number:
- 210-106-0
- EC Name:
- 2,6-dinitrotoluene
- Cas Number:
- 606-20-2
- Molecular formula:
- C7H6N2O4
- IUPAC Name:
- 2-methyl-1,3-dinitrobenzene
- Reference substance name:
- 3,4-dinitrotoluene
- EC Number:
- 210-222-1
- EC Name:
- 3,4-dinitrotoluene
- Cas Number:
- 610-39-9
- Molecular formula:
- C7H6N2O4
- IUPAC Name:
- 4-methyl-1,2-dinitrobenzene
- Details on test material:
- - Name of test material (as cited in study report):
2,3-Dinitrotoluol (2,3-DNT), CAS No. 602-01-7
2,4-Dinitrotoluol (2,4-DNT), CAS No. 121-14-2
2,6-Dinitrotoluol (2,6-DNT), CAS No. 606-20-2
3,4-Dinitrotoluol (3,4-DNT), CAS No. 610-39-9
- Analytical purity: no data
Constituent 1
Constituent 2
Constituent 3
Constituent 4
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- not specified
Test organisms
- Test organisms (species):
- Chlorella pyrenoidosa
- Details on test organisms:
- TEST ORGANISM
- Common name: Unicellular alga Chlorella pyrenoidosa
- Strain: Wisconsin 2005
- Source (laboratory, culture collection): Culture Centre of Algae and Protozoa, Cambridge
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 200 ml Erlenmeyer flask
- incubated on mechanical shaker
- Initial cells density: 10E+8 cells/L (100 mL)
GROWTH MEDIUM
- Algal medium according to van Leeuwen and Maas (1985)
OTHER TEST CONDITIONS
- Light intensity and quality: fluorescent light (7.5 W/m2)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Cells were counted with ZBI Coulter Counter using 70 μm aperture - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- 2,3-DNT
- Basis for effect:
- growth rate
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- 2,4-DNT
- Basis for effect:
- growth rate
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- 2,6-DNT
- Basis for effect:
- growth rate
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- 3,4-DNT
- Basis for effect:
- growth rate
Any other information on results incl. tables
Population growth:
log EC50 (µM) | EC50 (mg/L) | |
2,3 -DNT | 0.70 | 0.9 |
2,4 -DNT | 0.70 | 0.9 |
2,6 -DNT | 1.57 | 6.7 |
3,4 -DNT | 0.61 | 0.7 |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.