Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 201-247-9 | CAS number: 80-07-9
Tested for androgenic or anti-androgenic effects, DCDPS gave in vitro a negative response.
None of the tested compounds appeared to have any androgenic activity in the cell line up to the concentration of 50 µmol/L, when tested alone.
The development and optimization of a cell-based androgen reporter assay using the Chinese hamster ovarian cell line (CHO K1) in the 96 -well format is described. The intent is a use as screening system for identifying endocrine disrupting substances (ED) in a high throughput manner in environmental and biological samples. To this end, CHO cells were cotransfected with plasmids encoding mouse mammary tumour virus-neomycin-luciferase and human androgen receptor (hAR), and a stable cell line was established. After selection with neomycin, a highly active clone was obtained which stably expressed both the hAR and the androgen-responsive luciferase reporter. Stimulation of the cells with androgens for 24 h resulted in about 15-fold stimulation of luciferase activity, with the minimum effective dose of testosterone being 0.1 nmol/L. Potent steroidal and non-steroidal anti-androgens, such as hydroxyflutamide and cyproterone acetate, significantly inhibited the androgen-induced transactivation. Non-androgenic steroids like estradiol, progesterone, dexamethasone and cortisol showed weak activity at high concentrations. RT-PCR and western blot confirmed proper transcription and translation as well as stable expression of the AR gene in the cells.
About 60 different substances (mostly pesticides or their metabolites, and common industrial chemicals including DCDPS) were screened with the cell line for their ability to stimulate luciferase activity (indicating androgenic activity) or inhibit that evoked by 0.1 nmol/L R1881 (indicating anti-androgenic activity), used as a positive androgenic control. About 10 highly potent anti-androgenic chemicals were identified. The most potent anti-androgenic compounds identified included bisphenol A, α-hexachlorocyclohexane, vinclozolin and 4,4-DDE (CAS 72-55-9), a structural analogue to DCDPS, which gave a negative response. These compounds had alone either no effect or were weak agonists (with cytotoxic effects at very high concentrations), but none showed any significant agonistic activity. None of the tested compounds appeared to have any androgenic activity in the cell line up to the concentration of 50 µmol/L, when tested alone.
In conclusion, it is demonstrated the bioassay based on this cell line provides a reliable test for detecting androgenic and anti-androgenic compounds. DCDPS was not active as androgen or anti-androgen endocrine disruptor.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Close Do not show this message again