Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 232-565-6 | CAS number: 9000-90-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1995-02-13 to 1995-06-28
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Certificate included in the report
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Domestic waste water treatment plant : ARA Ergolz II, Füllinsdorf, Switzerland
- Preparation of inoculum for exposure: The sludge was washed three times with tap water and an amount corresponding to 4g/L dry material (± 10%) was mixed with Sörensen buffer solution pH7 and then aerated prior to incubation. An amount of 0.5 mL sludge (filtered over cotton wool) was added to 1000 mL test medium. - Duration of test (contact time):
- ca. 28 d
- Initial conc.:
- 44 mg/L
- Based on:
- test mat.
- Initial conc.:
- >= 18.2 - <= 19 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to OECD Guideline No. 301 E
- Test temperature: 19.7 - 21.5°C
- Continuous darkness: yes
TEST SYSTEM
- Number of culture flasks/concentration: 2
SAMPLING
- Sampling frequency: day 0 (treatment day) , 7, 14, 21, 27 and 28
- Sampling method: Per sampling interval, two flasks of the samples containing the test article or the reference compound, one flask of the inoculum blank and one flask of the toxicity control were taken and analysed for DOC in triplicate.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: Aniline and the test compound were dissolved in the test medium at concentrations of 45 mg/L SP 703 and 26 mg/L aniline corresponding to an actually measured concentration of 39.3 mg DOC/L - Reference substance:
- aniline
- Preliminary study:
- Test substance concentration was based on the DOC-values from a pre-test.
- Parameter:
- % degradation (DOC removal)
- Value:
- 99
- Sampling time:
- 28 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 95
- Sampling time:
- 7 d
- Results with reference substance:
- The reference compound aniline reached complete biodegradation during the first seven exposure days. At the end of the test, aniline was readily biodegraded by an average of 95.1%.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Alpha-amylase was found to be readily biodegradable in the 'Modified OECD Screening Test' (OECD 301E).
- Executive summary:
Alpha-amylase was tested for ready biodegradability in the '28 -day Modified OECD Screening Test'. The test was performed according to OECD 301E and in compliance with GLP.
The concentration of DOC (Disssolved Organic Carbon) in the test flasks containing alpha-amylase decreased by an average of 95.0 %, 92.5 % and 93.8% within 7, 14 and 21 days of exposure, respectively. At the end of the test (day 28), alpha-amylase was biodegraded by an average of 99.0%. Consequently, alpha-amylase was found to be readily biodegradable.
The reference compound Aniline reached complete biodegradation during the first seven exposure days. At the end of the test (day 28) Aniline was readily biodegraded by an average of 95.1%.
Reference
Description of key information
Alpha-amylase was found to be readily biodegradable in the 'Modified OECD Screening Test' (OECD 301E).
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Alpha-amylase was tested for ready biodegradability in the '28 -day Modified OECD Screening Test'. The test was performed according to OECD 301E and in compliance with GLP.
The concentration of DOC (Dissolved Organic Carbon) in the test flasks containing alpha-amylase decreased by an average of 95.0 %, 92.5 % and 93.8% within 7, 14 and 21 days of exposure, respectively. At the end of the test (day 28), alpha-amylase was biodegraded by an average of 99.0%. Consequently, alpha-amylase was found to be readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
